COL4A2-specific Polyclonal antibody

COL4A2-specific Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

69

Reviews (5)

Cat no : 55131-1-AP

Synonyms

COL4A2, Collagen Type IV, Collagen alpha-2(IV) chain, Collagen Ⅳ, Canstatin

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Validation Data Gallery

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  WB analysis using 55131-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55131-1-AP (COL4A2-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 55131-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55131-1-AP (Collagen Type IV antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 55131-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55131-1-AP (Collagen Type IV antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 55131-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55131-1-AP (Collagen Type IV antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 55131-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55131-1-AP (Collagen Type IV antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 55131-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 55131-1-AP (COL4A2-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 55131-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 55131-1-AP (COL4A2-specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55131-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55131-1-AP (Collagen Type IV antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55131-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55131-1-AP (Collagen Type IV antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human kidney using 55131-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using COL4A2-specific antibody (55131-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human kidney using 55131-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using COL4A2-specific antibody (55131-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse lung tissue, rat lung tissue
• Positive IHC detected in: human kidney tissue, human breast hyperplasia tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 22 publications below
• IF: See 13 publications below

Product Information

55131-1-AP targets COL4A2-specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: collagen, type IV, alpha 2
• Calculated Molecular Weight: 168 kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: NM_001846
• Gene Symbol: COL4A2
• Gene ID (NCBI): 1284
• RRID: AB_2881273
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
		EMBO J; Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress.; Authors - Simon Schwörer
human		EBioMedicine; Aspirin targets P4HA2 through inhibiting NF-κB and LMCD1-AS1/let-7g to inhibit tumour growth and collagen deposition in hepatocellular carcinoma.; Authors - Tianjiao Wang
	IF	ACS Appl Mater Interfaces; Novel Thermosensitive Hydrogel Promotes Spinal Cord Repair by Regulating Mitochondrial Function.; Authors - Yi Li
human	IF	Biomaterials; Tumor-specific nitric oxide generator to amplify peroxynitrite based on highly penetrable nanoparticles for metastasis inhibition and enhanced cancer therapy.; Authors - Menghao Shi
human	IHC	Biofabrication; Engineering microvasculature by 3D bioprinting of prevascularized spheroids in photo-crosslinkable gelatin.; Authors - Lise De Moor
	IHC	Biomed Pharmacother; Rutaecarpine alleviates inflammation and fibrosis by targeting CK2α in diabetic nephropathy; Authors - Juan Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Samantha (Verified Customer) (05-10-2024)

Clean bands detected in DRG and Spinal cord tissue of mice.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 1000 and 1 in 5000
• Cell Tissue Type: Dorsal Root Ganglion, Spinal Cord

FH

Pankhuri (Verified Customer) (08-22-2022)

Nice clean antibody. Did IF for collagen in NSCs organoid. The collagen fibres in the ECM of organoid were stained beautifully.

• Applications: Immunofluorescence
• Cell Tissue Type: NSC organoid

COL4A2-specific Antibody Immunofluorescence validation ( dilution) in NSC organoid (Cat no:55131-1-AP)

FH

Lindsey (Verified Customer) (05-24-2021)

BULLETPROOF ANTIBODY!

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human kidney (FFPE)

COL4A2-specific Antibody Immunohistochemistry,Immunofluorescence validation (1:500 dilution) in Human kidney (FFPE) (Cat no:55131-1-AP)

FH

Malak (Verified Customer) (02-24-2021)

This antibody works very well

• Applications: Western Blot

FH

Joshua (Verified Customer) (04-28-2019)

Vascular endothelial cells in 3D culture were fixed in paraformaldehdye and stained for collagen IV (red). DRAQ7 used as a counterstain (blue). As expected, collagen IV localized to the vascular basement membranes.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HMEC1

COL4A2-specific Antibody Immunofluorescence, validation (1:500 dilution) in HMEC1 (Cat no:55131-1-AP)