Mouse Tissue Factor Recombinant antibody, PBS Only (Capture/Detector)

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  WB analysis using 83887-4-RR (same clone as 83887-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83887-4-RR (Coagulation Factor III/Tissue Factor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83887-4-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 83887-4-RR (same clone as 83887-4-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 83887-4-RR (Coagulation Factor III/Tissue Factor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83887-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00859-1

  Sandwich ELISA standard curve of MP00859-1, Mouse Tissue Factor Recombinant Matched Antibody Pair - PBS only. 83887-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1102. 83887-4-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL.

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  Sample test of MP00859-1

  Urine of mice was measured. The Tissue Factor concentration of detected samples was determined to be 244.1 pg/mL.

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  Sample test of MP00859-1

  The mean Tissue Factor concentration was determined to be 18.4 ng/mL in mouse brain tissue extract based on a 6.0 mg/mL extract load, 13.1 ng/mL in C2C12 cell extract based on a 3.6 mg/mL extract load and 2.7 ng/mL in NIH/3T3 cell extract based on a 10.6 mg/mL extract load.

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  Sample test of MP00859-1

  Serum of sixteen mice was measured. The Tissue Factor concentration of detected samples was determined to be 86.6 pg/mL with a range of 63.0-105.0 pg/mL.

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  Cytometric bead array standard curve of MP00859-1

  Cytometric bead array standard curve of MP00859-1, MOUSE Tissue Factor Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83887-1-PBS. Detection antibody: 83887-4-PBS. Standard: Eg1102. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP00859-2

  Cytometric bead array standard curve of MP00859-2, MOUSE Tissue Factor Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83887-3-PBS. Detection antibody: 83887-4-PBS. Standard: Eg1102. Range: 0.313-20 ng/mL.

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  Cytometric bead array standard curve of MP00859-3

  Cytometric bead array standard curve of MP00859-3, MOUSE Tissue Factor Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83887-4-PBS. Detection antibody: 83887-2-PBS. Standard: Eg1102. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83887-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83887-4-RR against Mouse Tissue Factor were performed. The affinity constant is 0.85 nM.

Tissue Factor Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83887-4-PBS

Clone No.240875H3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD142, Coagulation factor III, F3, 240875H3, TF

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Product Information

83887-4-PBS targets Tissue Factor as part of a matched antibody pair:

MP00859-1: 83887-1-PBS capture and 83887-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00859-2: 83887-3-PBS capture and 83887-4-PBS detection (validated in Cytometric bead array)

MP00859-3: 83887-4-PBS capture and 83887-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Tissue Factor fusion protein Eg1102
• Full Name: coagulation factor III
• Calculated Molecular Weight: 33kDa
• Observed Molecular Weight: 48-53 kDa
• Gene Symbol: Tissue Factor
• Gene ID (NCBI): 14066
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P20352
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named Coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108). This antibody is specific for mouse Tissue Factor.