Cleaved PARP1 Monoclonal antibody, PBS Only (Capture)

Cleaved PARP1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA, Sample test

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

4G4C8

Cat no : 60555-1-PBS

Synonyms

PARP1, ARTD1, ADPRT1, ADPRT 1, ADPRT

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Validation Data Gallery

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  WB analysis using 60555-1-Ig (same clone as 60555-1-PBS)

  Staurosporine treated and untreated A2780 cells were subjected to SDS PAGE followed by western blot with 60555-1-Ig (Cleaved PARP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Lamin B1 (HRP-66095) antibody as a loading control. This data was developed using the same antibody clone with 60555-1-PBS in a different storage buffer formulation.

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  WB analysis using 60555-1-Ig (same clone as 60555-1-PBS)

  Staurosporine treated and untreated mouse splenocytes were subjected to SDS PAGE followed by western blot with 60555-1-Ig (Cleaved PARP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Lamin B1 (HRP-66095) antibody as a loading control. This data was developed using the same antibody clone with 60555-1-PBS in a different storage buffer formulation.

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  WB analysis using 60555-1-Ig (same clone as 60555-1-PBS)

  Staurosporine treated and untreated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60555-1-Ig (Cleaved PARP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Lamin B1 (HRP-66095) antibody as a loading control. This data was developed using the same antibody clone with 60555-1-PBS in a different storage buffer formulation.

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  Sample test of MP50985-2

  Cytometric bead array in cell lysate using MP50985-2, Cleaved PARP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60555-1-PBS. Detection antibody: 66520-1-PBS. Cell lysate: Non-treated Jurkat and Staurosporine treated Jurkat (10μg/well). Non-related target Tubulin-beta Recombinant Matched Antibody Pair (MP00550-1) was served as control.

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  IHC staining of Jurkat using 60555-1-Ig (same clone as 60555-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Jurkat (left) and Staurosporine treated Jurkat (right) cells slide using 60555-1-Ig (Cleaved PARP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60555-1-PBS in a different storage buffer formulation.

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  IF Staining of HSC-T6 using 60555-1-Ig (same clone as 60555-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed untreated and 1 μM Staurosporine (3 hours) treated HSC-T6 cells using Cleaved PARP1 antibody (60555-1-Ig, Clone: 4G4C8 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004). This data was developed using the same antibody clone with 60555-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60555-1-Ig (same clone as 60555-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed untreated and 1 μM Staurosporine (3 hours) treated HeLa cells using Cleaved PARP1 antibody (60555-1-Ig, Clone: 4G4C8 ) at dilution of 1:366 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60555-1-PBS in a different storage buffer formulation.

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  FC experiment of HSC-T6 using 60555-1-Ig (same clone as 60555-1-PBS)

  1x10^6 HSC-T6 cell (dash lines) and 1 μM  Staurosporine (3 hours) treated HSC-T6 cells (full lines) were intracellularly stained with 0.4 μg Cleaved PARP1 Monoclonal Antibody (60555-1-Ig, Clone:4G4C8, red) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 60555-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 60555-1-Ig (same clone as 60555-1-PBS)

  1x10^6 HeLa cell (dash lines) and 1 μM  Staurosporine (3 hours) treated HeLa cells (full lines) were intracellularly stained with 0.1 μg Cleaved PARP1 Monoclonal Antibody (60555-1-Ig, Clone:4G4C8, red) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 60555-1-PBS in a different storage buffer formulation.

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Product Information

60555-1-PBS targets Cleaved PARP1 as part of a matched antibody pair:

MP50985-2: 60555-1-PBS capture and 66520-1-PBS detection (validated in N/A)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: poly (ADP-ribose) polymerase 1
• Calculated Molecular Weight: 1014 aa, 113 kDa
• Observed Molecular Weight: 89 kDa
• GenBank Accession Number: BC037545
• Gene Symbol: PARP1
• Gene ID (NCBI): 142
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24 kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes, and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and give rise to fragments ranging from 42-89 kDa.
This antibody only recognizes the cleaved form of PAPR1 but not full-length PARP1.