Claudin 9 Polyclonal antibody, PBS Only

Claudin 9 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30520-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 30520-1-AP (same clone as 30520-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 30520-1-AP (Claudin 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30520-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 30520-1-AP (same clone as 30520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30520-1-AP (Claudin 9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30520-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 30520-1-AP (same clone as 30520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30520-1-AP (Claudin 9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30520-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 30520-1-AP (same clone as 30520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30520-1-AP (Claudin 9 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30520-1-PBS in a different storage buffer formulation.

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  IF Staining of T-47D using 30520-1-AP (same clone as 30520-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed T-47D cells using Claudin 9 antibody (30520-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 30520-1-PBS in a different storage buffer formulation.

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Product Information

30520-1-PBS targets Claudin 9 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Claudin 9 fusion protein Ag29607
• Full Name: claudin 9
• Calculated Molecular Weight: 217 aa, 23 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC051870
• Gene Symbol: Claudin 9
• Gene ID (NCBI): 9080
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium.They are small (20-27kDa) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. This antibody is specifically against claudin 9.