Claudin 5 Polyclonal antibody

Claudin 5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 29767-1-AP

Synonyms

CLDN5, CLDN 5, Claudin-5, Claudin5, BEC1

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Validation Data Gallery

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  WB analysis using 29767-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29767-1-AP (Claudin 5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 29767-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29767-1-AP (Claudin 5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 29767-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29767-1-AP (Claudin 5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: unboiled mouse lung tissue, unboiled rat lung tissue
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 11 publications below
• IF: See 1 publications below

Product Information

29767-1-AP targets Claudin 5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Claudin 5 fusion protein Ag29044
• Full Name: claudin 5
• Calculated Molecular Weight: 218 aa, 23 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC032363
• Gene Symbol: CLDN5
• Gene ID (NCBI): 7122
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 27 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.

Publications

Species	Application	Title
mouse	WB	Life Sci; Metabolomics study reveals increased deoxycholic acid contributes to deoxynivalenol-mediated intestinal barrier injury; Authors - Xin He
rat	WB	Int J Mol Sci; Ursolic Acid Alleviates Neuroinflammation after Intracerebral Hemorrhage by Mediating Microglial Pyroptosis via the NF-κB/NLRP3/GSDMD Pathway; Authors - Pan Lei
mouse	WB	Brain Res Bull; Inhibition of MMP-2 and MMP-9 attenuates surgery-induced cognitive impairment in aged mice; Authors - Yiqin Ji
rat	WB	Mol Biotechnol; Omega-3 Polyunsaturated Fatty Acids Alleviate Intestinal Barrier Dysfunction in Obstructive Jaundice Rats; Authors - Changxi Zhang
mouse	IF	Nan Fang Yi Ke Da Xue Xue Bao; [Aqueous extract of Chuan Xiong Rhizoma enhances inhibitory effect of temozolomide against brain metastasis of melanoma in mice]; Authors - Q Zhao
human	WB	iScience; A single cell atlas of circulating immune cells involved in diabetic retinopathy; Authors - Dan Liao