Claudin 1 Polyclonal antibody

Claudin 1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 28674-1-AP

Synonyms

claudin 1, CLD1, CLDN1, ILVASC, SEMP1



Tested Applications

Positive WB detected inA431 cells, mouse skin tissue, rat skin tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

28674-1-AP targets Claudin 1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivitymouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Claudin 1 fusion protein Ag29269
Full Name claudin 1
Calculated molecular weight 211 aa, 23 kDa
Observed molecular weight 20-23 kDa
GenBank accession numberBC012471
Gene symbol CLDN1
Gene ID (NCBI) 9076
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-1 is expressed primarily in keratinocytes and normal mammary epithelial cells.

Protocols

Product Specific Protocols
WB protocol for Claudin 1 antibody 28674-1-APDownload protocol
IHC protocol for Claudin 1 antibody 28674-1-APDownload protocol
IF protocol for Claudin 1 antibody 28674-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

Acta Pharmacol Sin

Hederacoside C ameliorates colitis via restoring impaired intestinal barrier through moderating S100A9/MAPK and neutrophil recruitment inactivation.

Authors - Zheng-Xia Zha
ratWB

Chin Med J (Engl)

Postconditioning of stellate ganglion block improves intestinal barrier function by inhibiting autophagy in conscious rats following hemorrhagic shock and resuscitation.

Authors - Zhonghua Li
ratWB

Bioengineered

Oxycodone protects cardiac microvascular endothelial cells against ischemia/reperfusion injury by binding to Sigma-1 Receptor.

Authors - Meihua Ji
ratIHC

Front Physiol

Gut Microbiota Was Involved in the Process of Liver Injury During Intra-Abdominal Hypertension.

Authors - Zeyu Zhao
mouseWB

Front Nutr

The Protective Effect of Sulforaphane on Dextran Sulfate Sodium-Induced Colitis Depends on Gut Microbial and Nrf2-Related Mechanism.

Authors - Canxia He
mouseWB

Int Immunopharmacol

Gastrodia remodels intestinal microflora to suppress inflammation in mice with early atherosclerosis.

Authors - Fei-Yu Liu