Chromogranin A Recombinant monoclonal antibody

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  WB analysis using 85605-2-RR

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 85605-2-RR (CHGA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85605-2-RR

  TT cells were subjected to SDS PAGE followed by western blot with 85605-2-RR (CHGA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85605-2-RR

  PC-12 cells were subjected to SDS PAGE followed by western blot with 85605-2-RR (CHGA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85605-2-RR

  Pig adrenal gland tissue was subjected to SDS PAGE followed by western blot with 85605-2-RR (CHGA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse pancreas using 85605-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 85605-2-RR (Chromogranin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendix using 85605-2-RR

  Immunohistochemical analysis of paraffin-embedded human appendix tissue slide using 85605-2-RR (Chromogranin A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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  IHC staining of mouse colon using 85605-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 85605-2-RR (Chromogranin A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 85605-2-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85605-2-RR (Chromogranin A antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 85605-2-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 85605-2-RR (CHGA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 85605-2-RR

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 85605-2-RR (CHGA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse pancreas using 85605-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Chromogranin A antibody (85605-2-RR, Clone: 243048H7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse small intestine using 85605-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Chromogranin A antibody (85605-2-RR, Clone: 243048H7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85605-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85605-2-RR against Human Chromogranin A were performed. The affinity constant is 3.39 nM.

Chromogranin A Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 85605-2-RR

Clone No.243048H7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

Synonyms

CGA, CHGA, AL-11, Chromogranin-A, EA-92

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, TT cells, PC-12 cells, pig adrenal gland tissue
• Positive IHC detected in: human pancreas tissue, human appendix tissue, human colon tissue, mouse colon tissue, mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse small intestine tissue, mouse pancreas tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85605-2-RR targets Chromogranin A in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag19780

  Product name: Recombinant human CHGA protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 19-457 aa of BC006459

  Sequence: LPVNSPMNKGDTEVMKCIVEVISDTLSKPSPMPVSQECFETLRGDERILSILRHQNLLKELQDLALQGAKERAHQQKKHSGFEDELSEVLENQSSQAELKEAVEEPSSKDVMEKREDSKEAEKSGEATDGARPQALPEPMQESKAEGNNQAPGEEEEEEEEATNTHPPASLPSQKYPGPQAEGDSEGLSQGLVDREKGLSAEPGWQAKREEEEEEEEEAEAGEEAVPEEEGPTVVLNPHPSLGYKEIRKGESRSEALAVDGAGKPGAEEAQDPEGKGEQEHSQQKEEEEEMAVVPQGLFRGGKSGELEQEEERLSKEWEDSKRWSKMDQLAKELTAEKRLEGQEEEEDNRDSSMKLSFRARAYGFRGPGPQLRRGWRPSSREDSLEAGLPLQVRGYPEEKKEEEGSANRRPEDQELESLSAIEAELEKVAHQLQALRRG

• Full Name: chromogranin A (parathyroid secretory protein 1)
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 70-85 kDa
• GenBank Accession Number: BC006459
• Gene Symbol: Chromogranin A
• Gene ID (NCBI): 1113
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P10645
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.