Ch-TOG Monoclonal antibody, PBS Only

Ch-TOG Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1H5G1

Cat no : 67631-1-PBS

Synonyms

ch TOG, CHTOG, Ch-TOG, CKAP5, FLJ35359, KIAA0097, MSPS, TOG, TOGp

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Validation Data Gallery

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  WB analysis using 67631-1-Ig (same clone as 67631-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67631-1-Ig (CKAP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67631-1-Ig (same clone as 67631-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67631-1-Ig (CKAP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67631-1-Ig (same clone as 67631-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ch-TOG antibody (67631-1-Ig, Clone: 1H5G1 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67631-1-PBS targets Ch-TOG in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Ch-TOG fusion protein Ag28770
• Full Name: cytoskeleton associated protein 5
• Observed Molecular Weight: 220-240 kDa
• GenBank Accession Number: BC120869
• Gene Symbol: CKAP5
• Gene ID (NCBI): 9793
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Ch-TOG (colonic hepatic tumor-overexpressed gene), also known as XMAP215 or CKAP5, is a microtubule polymerase which can promotes cytoplasmic microtubule nucleation and elongation. Through interacting with Aurora-A and TACC3, it plays a major role in organizing spindle poles.