Ch-TOG Monoclonal antibody, PBS Only (Capture/Detector)

Ch-TOG Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H5G1

Cat no : 67631-1-PBS

Synonyms

CKAP5, TOGp, Cytoskeleton-associated protein 5, Colonic and hepatic tumor overexpressed gene protein, ch TOG

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Validation Data Gallery

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  WB analysis using 67631-1-Ig (same clone as 67631-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67631-1-Ig (CKAP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

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  WB analysis using 67631-1-Ig (same clone as 67631-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67631-1-Ig (CKAP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 67631-1-Ig (same clone as 67631-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67631-1-Ig (Ch-TOG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 67631-1-Ig (same clone as 67631-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67631-1-Ig (Ch-TOG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 67631-1-Ig (same clone as 67631-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 67631-1-Ig (Ch-TOG antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 67631-1-Ig (same clone as 67631-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 67631-1-Ig (Ch-TOG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67631-1-Ig (same clone as 67631-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ch-TOG antibody (67631-1-Ig, Clone: 1H5G1 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50224-1

  Cytometric bead array standard curve of MP50224-1, CKAP5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67631-2-PBS. Detection antibody: 67631-1-PBS. Standard:Ag28770. Range: 0.391-100 ng/mL.

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  Cytometric bead array standard curve of MP50224-2

  Cytometric bead array standard curve of MP50224-2, CKAP5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67631-1-PBS. Detection antibody: 67631-3-PBS. Standard:Ag28770. Range: 6.25-100 ng/mL.

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Product Information

67631-1-PBS targets Ch-TOG as part of a matched antibody pair:

MP50224-1: 67631-2-PBS capture and 67631-1-PBS detection (validated in Cytometric bead array)

MP50224-2: 67631-1-PBS capture and 67631-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Ch-TOG fusion protein Ag28770
• Full Name: cytoskeleton associated protein 5
• Observed Molecular Weight: 220-240 kDa
• GenBank Accession Number: BC120869
• Gene Symbol: Ch-TOG
• Gene ID (NCBI): 9793
• RRID: AB_2882832
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Ch-TOG (colonic hepatic tumor-overexpressed gene), also known as XMAP215 or CKAP5, is a microtubule polymerase which can promotes cytoplasmic microtubule nucleation and elongation. Through interacting with Aurora-A and TACC3, it plays a major role in organizing spindle poles.