Cell Counting kit-8

Absorbance

450 nm

Application

Cell Counting

Cat no : PF00004



Product Information

Cell Counting Kit-8, based on WST-8 (chemical name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5- (2,4-disulfobenzene)- 2H-tetrazole monosodium salt), is a rapid and highlysensitive detection kit widely used in cell proliferation and cytotoxicity.In the presence of electron coupling reagent, WST-8 can be reduced by dehydrogenase in mitochondria to form orange formazan, which is highly water-soluble. The depth of color is directly proportional to cell proliferation and inversely proportional to cell toxicity. OD value was measured at 450 nm wavelength by enzyme labeling instrument, which indirectly reflected the number of living cells.

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WST-8 is an upgrade substitute product of MTT, which has obvious advantages compared with MTT or other similar products such as XTT or MTS. First of all, formazan, which is reduced by some dehydrogenase in mitochondria, is not water-soluble and needs to be dissolved in a specific solution; while formazan, produced by WST-8, XTT and MTS, is water-soluble, so subsequent dissolution steps can be omitted. Secondly, formazan produced by WST- 8 is more soluble than that produced by XTT and MTS. Thirdly, WST-8 is more stable than XTT and MTS, which makes the experimental results more stable. In addition, WST-8 has wider linear range and higher sensitivity than MTT and XTT.

Product     

100T            

500T            

3000T            

6000T          

 cck-8            

liquid          

Storage

It is effective for 2 years at 4℃ and 3 years at -20℃.

Protocol

CCK8 Protocol

Calculation

Cell viability=[(As-Ab)/(Ac-Ab)]×100% 

Inhibition rate=[(Ac-As)/(Ac-Ab)]×100% 

As:The absorbance of the experiment hole (medium containing cells, CCK-8, substance to be measured) Ac:Absorbance of control hole (medium containing cells, CCK-8, no substance to be measured) 

Ab:Blank (medium without cells and substance to be measured, CCK-8) absorbance

Cited in Article as

PF00004, Cell Counting kit-8, Proteintech, IL, USA

Publications

ApplicationTitle

Brief Bioinform

Ferroptosis-related lncRNA pairs to predict the clinical outcome and molecular characteristics of pancreatic ductal adenocarcinoma.

Authors - Rong Tang

Bioact Mater

TGF-β1-supplemented decellularized annulus fibrosus matrix hydrogels promote annulus fibrosus repair.

Authors - Qiang Wei

J Invest Dermatol

Chrna5 is overexpressed in psoriasis patients and promotes psoriasis-like inflammation in mouse models.

Authors - Jing Wang

Clin Transl Immunology

Retrospective analysis of the efficacy of adjuvant cytokine-induced killer cell immunotherapy combined with chemotherapy in colorectal cancer patients after surgery.

Authors - Xiao Li

Genomics

SHMT2 promotes the tumorigenesis of renal cell carcinoma by regulating the m6A modification of PPAT.

Authors - Fu-Chun Huo

J Enzyme Inhib Med Chem

The discovery of a novel series of potential ERRα inverse agonists based on p-nitrobenzenesulfonamide template for triple-negative breast cancer in vivo.

Authors - Zhipei Gao