CD2AP Polyclonal antibody

CD2AP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 24122-1-AP

Synonyms

METS-1, Mets1, METS 1, Mesenchyme-to-epithelium transition protein with SH3 domains 1, CD2-associated protein

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Validation Data Gallery

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  WB analysis using 24122-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24122-1-AP (Cd2ap antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 24122-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24122-1-AP (Cd2ap antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 24122-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24122-1-AP (Cd2ap antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse kidney tissue, K-562 cells
• Positive IHC detected in: mouse kidney tissue, mouse stomach tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:400-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

24122-1-AP targets CD2AP in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD2AP fusion protein Ag21170
• Full Name: CD2-associated protein
• Calculated Molecular Weight: 637 aa, 70 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC157901
• Gene Symbol: Cd2ap
• Gene ID (NCBI): 12488
• RRID: AB_2879436
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD2 associated protein (CD2AP, also known as CMS) is a scaffolding molecule that acts as an adapter protein between membrane proteins and the actin cytoskeleton (PMID: 10339567). CD2AP also plays a role in epithelial cell junction formation and affects the pathogenesis of Alzheimer's disease (PMID: 22891260; 31440393). Its mutations are associated with sporadic nephrotic syndrome and focal segmental glomerulosclerosis (FSGS) (PMID: 17713465; 19131354).

Publications

Species	Application	Title
human	WB	Front Med (Lausanne); MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene.; Authors - Hui Song
mouse	WB	Am J Physiol Cell Physiol; Long non-coding RNA Kcnq1ot1 promotes sC5b-9-induced podocyte pyroptosis by inhibiting miR-486a-3p and upregulating NLRP3.; Authors - Chunjian Zhang
mouse	WB	Inflammation; Fractalkine is Involved in Lipopolysaccharide-Induced Podocyte Injury through the Wnt/β-Catenin Pathway in an Acute Kidney Injury Mouse Model.; Authors - Soulixay Senouthai