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Cathepsin L Polyclonal antibody

Cathepsin L Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27952-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Cathepsin L, cathepsin L1, CATL, CTSL, CTSL1, FLJ31037, Major excreted protein, MEP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27952-1-AP targets Cathepsin L in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cathepsin L fusion protein Ag27813 Predict reactive species
Full Name cathepsin L1
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 36-39 kDa, 29 kDa
GenBank Accession NumberBC012612
Gene Symbol Cathepsin L
Gene ID (NCBI) 1514
RRIDAB_2881021
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07711
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTSL1(Cathepsin L1) is also named as CTSL and MEP, belongs to the peptidase C1 family. It is a lysosomal proteinase whose expression is also up-regulated in the skeletal muscle during starvation(PMID:20088826). It plays an intracellular role in normal intestinal epithelial polarization and initiation of neoplasia(PMID:17622569). CTSL1 also improves cardiac function and inhibits cardiac hypertrophy, inflammation, and fibrosis through blocking AKT/GSK3B signaling(PMID:19096818). The full length protein is 38 kDa with a signal peptide, two propeptide and a glycosylation site. It has been detected the 36 kDa, 39 kDa, 29 kDa and 21 kDa in rat gastrocnemius muscle. These forms of cathepsin L could either be attributed to differences in glycosylation or to partial processing of the proenzyme. (PMID:11696001)

Protocols

Product Specific Protocols
WB protocol for Cathepsin L antibody 27952-1-APDownload protocol
IHC protocol for Cathepsin L antibody 27952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Front Pharmacol

Nodosin Exerts an Anti-Colorectal Cancer Effect by Inhibiting Proliferation and Triggering Complex Cell Death in Vitro and in Vivo

Authors - Huixia Fan
humanWB

Acta Pharmacol Sin

Salvianolic acid B suppresses hepatic fibrosis by inhibiting ceramide glucosyltransferase in hepatic stellate cells

Authors - Zi-Bo Li
humanIHC

Transl Oncol

Mitochondria-related chemoradiotherapy resistance genes-based machine learning model associated with immune cell infiltration on the prognosis of esophageal cancer and its value in pan-cancer

Authors - Ziyu Liu
mouseWB

J Med Chem

Design, Synthesis, and Biological Evaluation of 2-Arylaminopyrimidine Derivatives as Dual Cathepsin L and JAK Inhibitors for the Treatment of Acute Lung Injury

Authors - Chunwei Shen
humanWB

Pharmacol Res

The molecular receptor NKBB enhances the persistence and anti-hepatocellular carcinoma activity of GPC3 CAR-T cells

Authors - Minghao Sui
humanWB,IHC,IF

World J Gastroenterol

Macrophage-derived cathepsin L promotes epithelial-mesenchymal transition and M2 polarization in gastric cancer

Authors - Lu-Xi Xiao
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