Cathepsin D Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Cathepsin D Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2F6F7

Cat no : 66534-1-PBS

Synonyms

CTSD, CPSD, CLN10, Cathepsin D light chain, Cathepsin D heavy chain

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Validation Data Gallery

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  WB analysis using 66534-1-Ig (same clone as 66534-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66534-1-Ig (Cathepsin D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66534-1-Ig (same clone as 66534-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66534-1-Ig (Cathepsin D antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66534-1-Ig (same clone as 66534-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66534-1-Ig (same clone as 66534-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66534-1-Ig (same clone as 66534-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66534-1-Ig (same clone as 66534-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66534-1-Ig (same clone as 66534-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66534-1-Ig (same clone as 66534-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66534-1-Ig (same clone as 66534-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cathepsin D antibody (66534-1-Ig, Clone: 2F6F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66534-1-Ig (same clone as 66534-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Cathepsin D (66534-1-Ig, Clone:2F6F7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

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Product Information

66534-1-PBS targets Cathepsin D in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cathepsin D fusion protein Ag15254
• Full Name: cathepsin D
• Calculated Molecular Weight: 412 aa, 45 kDa
• Observed Molecular Weight: 32 kDa, 48 kDa, 52 kDa
• GenBank Accession Number: BC016320
• Gene Symbol: Cathepsin D
• Gene ID (NCBI): 1509
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CTSD (Cathepsin D) is also named as CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. (PMID: 27114232, PMID: 30717773, PMID: 30051532)