Cathepsin D Polyclonal antibody

Cathepsin D Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31516-1-AP

Synonyms

CTSD, EC:3.4.23.5, CPSD, CLN10, Cathepsin D light chain

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Validation Data Gallery

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  WB analysis using 31516-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31516-1-AP (Cathepsin D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 31516-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31516-1-AP (Cathepsin D antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 31516-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31516-1-AP (Cathepsin D antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 31516-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cathepsin D antibody (31516-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of MCF-7 using 31516-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Cathepsin D antibody (31516-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells, HuH-7 cells, MCF-7 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31516-1-AP targets Cathepsin D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: cathepsin D
• Calculated Molecular Weight: 45kDa
• Observed Molecular Weight: 32 kDa, 48 kDa, 52 kDa
• GenBank Accession Number: NM_001909.5
• Gene Symbol: Cathepsin D
• Gene ID (NCBI): 1509
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTSD (cathepsin D) is a lysosomal aspartic protease in the pepsin superfamily ubiquitous to all cell types but particularly abundant in the central nervous system (CNS). The signal peptide of the pre-proCTSD (52 kDa) is cleaved in the endoplasmic reticulum (ER), forming the pro-CTSD (48 kDa), finally processed to form the mature CTSD (32 kDa) in the lysosome (PMID: 31282275,15126630). The major role of the m-CTSD is the digestion of internalized waste cell proteins and peptides in the lysosome, which maintains cell health (PMID: 32324083). CTSD is a well-established aspartyl protease that functions intracellularly in lysosomes and extracellularly in several forms of cancer.