Cathepsin C Polyclonal antibody

Cathepsin C Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30790-1-AP

Synonyms

CTSC, CPPI

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Validation Data Gallery

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  WB analysis using 30790-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30790-1-AP (Cathepsin C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin cancer using 30790-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 30790-1-AP (Cathepsin C antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 30790-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using Cathepsin C antibody (30790-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30790-1-AP targets Cathepsin C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cathepsin C fusion protein Ag32420
• Full Name: cathepsin C
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: NM_001814
• Gene Symbol: Cathepsin C
• Gene ID (NCBI): 1075
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cathepsin C, also known as DPP-I. It is expected to be located in lysosome and highly expressed in lung, kidney and placenta (PMID: 9092576). This gene encodes a member of the peptidase C1 family and lysosomal cysteine proteinase that appears to be a central coordinator for activation of many serine proteinases in cells of the immune system. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate heavy and light chains that form a disulfide-linked dimer. Defects in the encoded protein have been shown to be a cause of Papillon-Lefevre syndrome, an autosomal recessive disorder characterized by palmoplantar keratosis and periodontitis. The calculated molecular weight of Cathepsin C is 51 kDa. The 23-kDa band may be a subunit of the protein monomer, while a 100-kda band is a dimer form (PMID: 26884336, 31557781).