Caspase 9/p35/p10 Monoclonal antibody, PBS Only (Capture)

Caspase 9/p35/p10 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B7G2

Cat no : 66169-1-PBS

Synonyms

CASP9, Caspase 9, Apoptotic protease Mch-6, Apoptotic protease Mch 6, APAF-3

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Validation Data Gallery

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  WB analysis of Jurkat using 66169-1-Ig (same clone as 66169-1-PBS)

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66169-1-Ig (Caspase 9/p35/p10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66169-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66169-1-Ig (same clone as 66169-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66169-1-Ig (Caspase 9/p35/p10 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66169-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 66169-1-Ig (same clone as 66169-1-PBS)

  IP result of anti-Caspase 9/p35/p10 (IP:66169-1-Ig, 5ug; Detection:66169-1-Ig 1:500) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 66169-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66169-1-Ig (same clone as 66169-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66169-1-Ig (Caspase 9/p35/p10 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66169-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66169-1-Ig (same clone as 66169-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66169-1-Ig (Caspase 9/p35/p10 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66169-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 66169-1-Ig (same clone as 66169-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66169-1-Ig (Caspase 9/p35/p10 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66169-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 66169-1-Ig (same clone as 66169-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66169-1-Ig (Caspase 9/p35/p10 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66169-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66169-1-Ig (same clone as 66169-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66169-1-Ig(Caspase 9/p35/p10 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66169-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50283-1

  Cytometric bead array standard curve of MP50283-1, Caspase 9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66169-1-PBS. Detection antibody: 66169-2-PBS. Standard:Ag20813. Range: 1.563-200 ng/mL.

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Product Information

66169-1-PBS targets Caspase 9/p35/p10 as part of a matched antibody pair:

MP50283-1: 66169-1-PBS capture and 66169-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Caspase 9/p35/p10 fusion protein Ag20813
• Full Name: caspase 9, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa, 35 kDa
• GenBank Accession Number: BC002452
• Gene Symbol: Caspase 9
• Gene ID (NCBI): 842
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Caspase 9, apoptosis-related cysteine protease (CASP9,synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Capase 9 is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. In recent years, the localization of caspase9 was a focus of interest. Beside its cytoplasmic distribution, a very extensive localization study was done on rat brain tissue, where caspase9 was found located predominantly in the nucleus and to a lesser extend in the cytoplasm [PMID: 15541731].