Caspase 6 Monoclonal antibody

• Featured Product
• KD/KO Validated

Caspase 6 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1H7F9

Cat no : 67825-1-Ig

Synonyms

Apoptotic protease Mch 2, CASP 6, CASP6, Caspase 6, Caspase6, MCH2

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Validation Data Gallery

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  WB analysis using 67825-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67825-1-Ig (Caspase 6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67825-1-Ig

  WB result of Caspase 6 antibody (67825-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 6 transfected Jurkat cells.

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  IF Staining of Jurkat using 67825-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Caspase 6 antibody (67825-1-Ig, Clone: 1H7F9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

67825-1-Ig targets Caspase 6 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Caspase 6 fusion protein Ag20534
• Full Name: caspase 6, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 33 kDa, 22 kDa
• Observed Molecular Weight: 33-35 kDa，22kDa, 18kDa
• GenBank Accession Number: BC000305
• Gene Symbol: Caspase 6
• Gene ID (NCBI): 839
• RRID: AB_2918587
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase-6 belongs to caspase family of cysteinyl-aspartate specific proteases.Precursor of CASP6 produces two subunits, large (18kDa) and small (16kDa) that dimerize. It cleaves poly(ADP-ribose) polymerase, as well as lamins and is involved in the activation cascade of caspases responsible for apoptosis execution. Researches showed that CASP6 could be an early instigator of neuronal dysfunction and regulates B cell activation and differentiation into plasma cells by modifying cell cycle entry. IRAK3 is an important target for CASP6. It can reveal five bands of 28, 32, 36, 49, and 64 kDa in human neurons and fetal brain in western blot, the 32 and 28 kDa bands represent procaspase-6 and pro-arm caspase-6. Procaspase-6 is more abundant than pro-arm caspase-6 in adult tissue, whereas pro-arm caspase-6 is more abundant than pro-caspase-6 in fetal brain and cultured neurons. The higher molecular mass bands at 49 and 64 kDa likely represent dimers of p28 and p32.(PMID:10438520). In rat testis, it can be detected two bands of 34 kDa and 12 kDa or 14 kDa(PMID:12538628).

Publications

Species	Application	Title
human	WB	Theranostics; Caspase-mediated AURKA cleavage at Asp132 is essential for paclitaxel to elicit cell apoptosis; Authors - Xiaoting Chen ; KO Validated
human	WB	Apoptosis; Strong activation of p53 by actinomycin D and nutlin-3a overcomes the resistance of cancer cells to the pro-apoptotic activity of the FAS ligand; Authors - Barbara Łasut-Szyszka