Caspase 3 Recombinant antibody, PBS Only

Caspase 3 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5G20

Cat no : 82202-1-PBS

Synonyms

CASP3, Caspase3, Caspase-3, CASP-3, CASP 3

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 82202-1-RR (same clone as 82202-1-PBS)

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 82202-1-RR (Caspase 3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 82202-1-RR (same clone as 82202-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82202-1-RR (Caspase 3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 82202-1-RR (same clone as 82202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 82202-1-RR (same clone as 82202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 82202-1-RR (same clone as 82202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 82202-1-RR (same clone as 82202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 82202-1-RR (same clone as 82202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 82202-1-RR (same clone as 82202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 82202-1-RR (same clone as 82202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 82202-1-RR (same clone as 82202-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 3 antibody (82202-1-RR, Clone: 5G20 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 82202-1-RR (same clone as 82202-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 3 antibody (82202-1-RR, Clone: 5G20 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 82202-1-RR (same clone as 82202-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Caspase 3 (82202-1-RR, Clone:5G20) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82202-1-PBS in a different storage buffer formulation.

---

Product Information

82202-1-PBS targets Caspase 3 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: caspase 3, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32-35 kDa, 17 kDa, 19 kDa
• GenBank Accession Number: NM_004346
• Gene Symbol: Caspase 3
• Gene ID (NCBI): 836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.