Caspase 3/p17/p19 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Caspase 3/p17/p19 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2G4B2

Cat no : 66470-2-PBS

Synonyms

CASP3, Caspase3, Caspase 3, CASP-3, CASP 3

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 66470-2-Ig (same clone as 66470-2-PBS)

  WB result of Caspase 3 antibody (66470-2-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 3 transfected HEK-293 cells. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66470-2-Ig (same clone as 66470-2-PBS)

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66470-2-Ig (same clone as 66470-2-PBS)

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 66470-2-Ig (same clone as 66470-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66470-2-Ig (Caspase 3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66470-2-Ig (same clone as 66470-2-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66470-2-Ig (same clone as 66470-2-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 66470-2-Ig (same clone as 66470-2-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66470-2-Ig (CASP3 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66470-2-Ig (CASP3 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Caspase 3/p17/p19 antibody (66470-2-Ig, Clone: 2G4B2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

---

Product Information

66470-2-PBS targets Caspase 3/p17/p19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Caspase 3/p17/p19 fusion protein Ag25029
• Full Name: caspase 3, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 277 aa, 32 kDa
• Observed Molecular Weight: 32-35 kDa, 19 kDa, 17 kDa
• GenBank Accession Number: BC016926
• Gene Symbol: Caspase 3
• Gene ID (NCBI): 836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.