Caspase 2/p18 Polyclonal antibody

Caspase 2/p18 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24777-1-AP

Synonyms

CASP 2, CASP2, Caspase 2, Caspase 2/p18, Caspase2, ICH 1L, ICH 1L/1S, ICH1, NEDD 2, NEDD2, Protease ICH 1

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Validation Data Gallery

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  WB analysis of HEK-293 using 24777-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24777-1-AP (Caspase 2/p18 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 24777-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24777-1-AP (Caspase 2/p18 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 24777-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24777-1-AP (Caspase 2/p18 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 24777-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24777-1-AP (Caspase 2/p18 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24777-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24777-1-AP (Caspase 2/p18 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 24777-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24777-1-AP (Caspase 2/p18 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 24777-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24777-1-AP (Caspase 2/p18 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 24777-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 24777-1-AP (Caspase 2/p18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, mouse kidney tissue
• Positive IHC detected in: human liver cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24777-1-AP is Caspase 2/p18 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Caspase 2/p18 fusion protein Ag20141
• Full Name: caspase 2, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 452 aa, 51 kDa
• Observed Molecular Weight: 48-51 kDa, 32 kDa
• GenBank Accession Number: BC002427
• Gene Symbol: Caspase 2
• Gene ID (NCBI): 835
• RRID: AB_2879715
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.It is involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.It has 2 isoforms produced by alternative splicing and can exists almost as a dimer in solution(PMID:15865942).