Cas9 antibody (mAb) (Clone 7A9-3A3)

Host / Isotype

Mouse / IgG1k

Reactivity

Human, Not Species Specific

Applications

ChIP, ICC, IF, IP, WB

CloneNo.

7A9-3A3

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Cat No : 61577,61578,61978 61577

Synonyms

Cas9, Streptococcus pyogenes, CRISPR, clustered regularly interspaced short palindromic repeat, gene-editing, guide RNA, nuclease, endonuclease, targeting, DNA, double-strand break, RNA, genome editing, wb, ip, if, western blotting, immunoprecipitation, immunoflourescence, antibody, antibodies, mab, monoclonal, sample



Product Information

Tested Applications ChIP, ICC, IF, IP, WB

Applications Validated by Active Motif: IP: 5 ug per IP ICC/IF: 0.5 - 2 ug/ml dilution WB: 0.1 - 1 ug/ml dilution

Tested Reactivity Human, Not Species Specific
Host / Isotype Mouse / IgG1k
Class Monoclonal
Type Antibody
Immunogen This antibody was raised against a recombinant protein within the N-terminal region of Streptococcus pyogene Cas9. This antibody should recognize Cas9 and dCas9 based on the antigen design.
Full Name Cas9 antibody (mAb) (Clone 7A9-3A3)
Synonyms Cas9, Streptococcus pyogenes, CRISPR, clustered regularly interspaced short palindromic repeat, gene-editing, guide RNA, nuclease, endonuclease, targeting, DNA, double-strand break, RNA, genome editing, wb, ip, if, western blotting, immunoprecipitation, immunoflourescence, antibody, antibodies, mab, monoclonal, sample
Molecular weight 160 kDa
GenBank accession numberWP_014407541
RRIDAB_2793684
Purification Method Protein G Chromatography
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Cas9 is a nuclease from Streptococcus pyogenes that can be targeted to particular DNA sequences through a guide RNA that results in double-stranded breaks in DNA. Cas9 is part of the CRISPR/Cas9 gene-editing system that can create a DNA break at a specific location with the genome. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) Probable. In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.