Carboxypeptidase A1 Polyclonal antibody, PBS Only

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  WB analysis of mouse pancreas using 15836-1-AP (same clone as 15836-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse pancreas using 15836-1-AP (same clone as 15836-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 15836-1-AP (same clone as 15836-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 15836-1-AP (same clone as 15836-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 15836-1-AP (same clone as 15836-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 15836-1-AP (same clone as 15836-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 15836-1-AP (same clone as 15836-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 15836-1-AP (same clone as 15836-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 15836-1-AP (same clone as 15836-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 15836-1-AP (same clone as 15836-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 15836-1-AP (same clone as 15836-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 15836-1-AP (same clone as 15836-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 15836-1-AP (same clone as 15836-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Carboxypeptidase A1 antibody (15836-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15836-1-PBS in a different storage buffer formulation.

Carboxypeptidase A1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 15836-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CPA1, EC:3.4.17.1, CPA

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Product Information

15836-1-PBS targets Carboxypeptidase A1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Carboxypeptidase A1 fusion protein Ag8586
• Full Name: carboxypeptidase A1 (pancreatic)
• Calculated Molecular Weight: 419 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC005279
• Gene Symbol: Carboxypeptidase A1
• Gene ID (NCBI): 1357
• RRID: AB_2085586
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P15085
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

CPA1(Carboxypeptidase A1) is also named as CPA and belongs to the the peptidase M14 family. CPA1 is one of the genes whose expression in the pancreas and the protein which it encodes is synthesized as an inactive precursor, proCPA1, which is processed to the active enzyme by the proteolytic removal of the 95-amino acid N-terminal prodomain. The mature CPA1 is not secreted and is trapped and degraded intracellularly(PMID:8639628). This antibody is speicific to CPA1.