Cannabinoid receptor 2 Polyclonal antibody

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  WB analysis of MCF-7 using 29371-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 29371-1-AP (Cannabinoid receptor 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 29371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29371-1-AP (Cannabinoid receptor 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 29371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29371-1-AP (Cannabinoid receptor 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Cannabinoid receptor 2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 29371-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

CNR2, CB2B, CB2A, CB-2, CB2

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

29371-1-AP targets Cannabinoid receptor 2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cannabinoid receptor 2 fusion protein Ag30609
• Full Name: cannabinoid receptor 2 (macrophage)
• Calculated Molecular Weight: 360 aa, 40 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC074767
• Gene Symbol: Cannabinoid receptor 2
• Gene ID (NCBI): 1269
• RRID: AB_2918285
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P34972
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cannabinoid receptor 1 (CNR1, or CB1) and CNR2 (CB2) are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family. The CB1 receptor is expressed mainly in the brain. The CB2 receptor is expressed mainly in the immune system and in hematopoietic cells. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana.

Publications

Species	Application	Title
mouse	WB	World J Gastroenterol; Role of gut microbiota in Crohn's disease pathogenesis: Insights from fecal microbiota transplantation in mouse model; Authors - Qiang Wu