Calpain 7 Polyclonal antibody

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  WB analysis of mouse brain using 26985-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26985-1-AP (Calpain 7 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26985-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26985-1-AP (Calpain 7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26985-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26985-1-AP (Calpain 7 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 26985-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 26985-1-AP (Calpain 7 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

Calpain 7 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26985-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

CAPN7, PalBH, EC:3.4.22.-, Calpain-7, CALPAIN7

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, rat brain tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Product Information

26985-1-AP targets Calpain 7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Calpain 7 fusion protein Ag25574
• Full Name: calpain 7
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 92 kDa
• GenBank Accession Number: BC056202
• Gene Symbol: Calpain 7
• Gene ID (NCBI): 23473
• RRID: AB_2880713
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y6W3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain 7 (also known as PalBH) is a mammalian homologue of the Aspergillus, atypical calpain PalB. It is a unique intracellular cysteine protease that has a tandem repeat of microtubule interacting and trafficking (MIT) domains and lacks a penta-EF-hand domain.

Publications

Species	Application	Title
mouse	WB	EMBO Mol Med; CRTH2 promotes endoplasmic reticulum stress-induced cardiomyocyte apoptosis through m-calpain.; Authors - Shengkai Zuo
human	WB,IF	Elife; Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission; Authors - Dawn M Wenzel
human	WB,IF	Elife; The Calpain-7 protease functions together with the ESCRT-III protein IST1 within the midbody to regulate the timing and completion of abscission; Authors - Elliott L Paine ; KD Validated