Calpain 3 Polyclonal antibody, PBS Only

Calpain 3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10415-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis of mouse skeletal muscle using 10415-1-AP (same clone as 10415-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10415-1-AP (Calpain 3 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10415-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 10415-1-AP (same clone as 10415-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10415-1-AP (Calpain 3 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10415-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human urothelial carcinoma using 10415-1-AP (same clone as 10415-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10415-1-AP (Calpain 3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10415-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human urothelial carcinoma using 10415-1-AP (same clone as 10415-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10415-1-AP (Calpain 3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10415-1-PBS in a different storage buffer formulation.

---

Product Information

10415-1-PBS targets Calpain 3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Calpain 3 fusion protein Ag0671
• Full Name: calpain 3, (p94)
• Calculated Molecular Weight: 94 kDa
• Observed Molecular Weight: 90-105 kDa, 55-60 kDa, 30-35 kDa
• GenBank Accession Number: BC003521
• Gene Symbol: CAPN3
• Gene ID (NCBI): 825
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Calpain 3 (CAPN3), a major intracellular protease, is a muscle-specific member of the calpain large subunit family that specifically binds to titin. CAPN3 plays a role in the dysferlin protein complex and that disruption of CAPN3 function may affect muscle membrane repair and remodeling. CAPN3 has some isoforms with MW of 94, 84, 93, 36, 18 kDa. CAPN3 autolysis generates a small N-terminal fragment of 34 kDa and a large C-terminal fragment whose size ranges from 55 to 60 kDa during self-processing (PMID: 9794799, 14645524).