Calnexin Recombinant antibody, PBS Only

Calnexin Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3K18

Cat no : 81938-1-PBS

Synonyms

calnexin, CANX, CNX, FLJ26570, IP90, P90

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Validation Data Gallery

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  WB analysis using 81938-1-RR (same clone as 81938-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81938-1-RR (Calnexin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81938-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 81938-1-RR (same clone as 81938-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 81938-1-RR (Calnexin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81938-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 81938-1-RR (same clone as 81938-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 81938-1-RR (Calnexin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81938-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 81938-1-RR (same clone as 81938-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Calnexin antibody (81938-1-RR, Clone: 3K18 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81938-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 81938-1-RR (same clone as 81938-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Calnexin antibody (81938-1-RR, Clone: 3K18 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81938-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81938-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 81938-1-RR against Human Calnexin were performed. The affinity constant is 1.76 nM.

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Product Information

81938-1-PBS targets Calnexin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Calnexin fusion protein Ag0697
• Full Name: calnexin
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC003552
• Gene Symbol: Calnexin
• Gene ID (NCBI): 821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.