Calnexin Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Calnexin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A2C6

Cat no : 66903-1-PBS

Synonyms

calnexin, CANX, CNX, FLJ26570, IP90, P90

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Validation Data Gallery

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  WB analysis using 66903-1-Ig (same clone as 66903-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66903-1-Ig (Calnexin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66903-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66903-1-Ig (same clone as 66903-1-PBS)

  WB result of Calnexin antibody (66903-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calnexin transfected HEK-293 cells. This data was developed using the same antibody clone with 66903-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66903-1-Ig (same clone as 66903-1-PBS)

  HEK-293 cells and HEK-293-derived exosomes (HEK-293-exo) were subjected to SDS PAGE followed by western blot with 66903-1-Ig (Calnexin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66903-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 66903-1-Ig (same clone as 66903-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 66903-1-Ig (Calnexin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66903-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 66903-1-Ig (same clone as 66903-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 66903-1-Ig (Calnexin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66903-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66903-1-Ig (same clone as 66903-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66903-1-Ig (Calnexin antibody), at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). Red: staning with CoraLite555-Phalloidin. This data was developed using the same antibody clone with 66903-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66903-1-Ig (same clone as 66903-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66903-1-Ig (Calnexin antibody) at dilution of 1:200 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66903-1-PBS in a different storage buffer formulation.

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Product Information

66903-1-PBS targets Calnexin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Calnexin fusion protein Ag25535
• Full Name: calnexin
• Calculated Molecular Weight: 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC003552
• Gene Symbol: Calnexin
• Gene ID (NCBI): 821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Calnexin is a molecular chaperone that resides in the endoplasmic reticulum (ER) and participates in the folding and assembly of newly synthesized proteins. Calnexin is highly abundant in ER and is frequently used as an ER marker.