Cytohesin 2 Polyclonal antibody

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  WB analysis of mouse heart using 10405-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10405-1-AP (Cytohesin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10405-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10405-1-AP (Cytohesin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 10405-1-AP

  IP result of anti-Cytohesin 2 (IP:10405-1-AP, 4ug; Detection:10405-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of human colon using 10405-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10405-1-AP (Cytohesin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 10405-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10405-1-AP (Cytohesin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 10405-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10405-1-AP (Cytohesin 2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 10405-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10405-1-AP (Cytohesin 2 antibody) at dilution of 1:200 (under 40x lens).

• Featured Product
• KD/KO Validated

Cytohesin 2 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 10405-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Synonyms

ARF exchange factor, ARNO, CTS18, CTS18.1, CYTH2, cytohesin 2, Protein ARNO, PSCD2, PSCD2L, SEC7L, Sec7p L, Sec7p like

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Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human colon tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

10405-1-AP targets Cytohesin 2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytohesin 2 fusion protein Ag0687
• Full Name: cytohesin 2
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC004361
• Gene Symbol: Cytohesin 2
• Gene ID (NCBI): 9266
• RRID: AB_2089028
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99418
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pleckstrin homology, Sec7 and coiled/coil domains 2 (PSCD2) is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. PSCD2 exhibits GEP activity in vitro with small G proteins ARF1, ARF3, and ARF6.

Publications

Species	Application	Title
rat	WB, IF	J Neurosci; Inhibition of Cytohesins Protects against Genetic Models of Motor Neuron Disease.; Authors - Jinbin Zhai
mouse	WB,IHC	J Biol Chem; The synaptic scaffolding protein CNKSR2 interacts with CYTH2 to mediate hippocampal granule cell development.; Authors - Hidenori Ito ; KD Validated
human	WB	Oncol Rep; Cytohesin-2 as a novel prognostic marker for hepatocellular carcinoma.; Authors - Xu Kedong K
rat	WB,IP	Open Biol; ARNO is recruited by the neuronal adaptor FE65 to potentiate ARF6-mediated neurite outgrowth; Authors - Yuqi Zhai