CYR61 Monoclonal antibody, PBS Only

CYR61 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E11F11

Cat no : 67656-1-PBS

Synonyms

CCN family member 1, CCN1, CYR61, GIG1, IBP 10, IGF binding protein 10, IGFBP 10, IGFBP10, Protein CYR61, Protein GIG1

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Validation Data Gallery

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  WB analysis using 67656-1-Ig (same clone as 67656-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67656-1-Ig (CYR61 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67656-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67656-1-Ig (same clone as 67656-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67656-1-Ig (CYR61 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67656-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67656-1-Ig (same clone as 67656-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67656-1-Ig (CYR61 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67656-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 67656-1-Ig (same clone as 67656-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67656-1-Ig (CYR61 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67656-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 67656-1-Ig (same clone as 67656-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67656-1-Ig (CYR61 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67656-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 67656-1-Ig (same clone as 67656-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CYR61 antibody (67656-1-Ig, Clone: 2E11F11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67656-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 67656-1-Ig (same clone as 67656-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CYR61 antibody (67656-1-Ig, Clone: 2E11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67656-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67656-1-PBS targets CYR61 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CYR61 fusion protein Ag25129
• Full Name: cysteine-rich, angiogenic inducer, 61
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 40-42 kDa
• GenBank Accession Number: BC001271
• Gene Symbol: CYR61
• Gene ID (NCBI): 3491
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.