CYP7B1 Polyclonal antibody

CYP7B1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24889-1-AP

Synonyms

CBAS3, CP7B, CYP7B1, Cytochrome P450 7B1, Oxysterol 7 alpha hydroxylase, SPG5A



Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human brain tissue, human liver tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24889-1-AP targets CYP7B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CYP7B1 fusion protein Ag19374
Full Name cytochrome P450, family 7, subfamily B, polypeptide 1
Calculated Molecular Weight 506 aa, 58 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC136574
Gene Symbol CYP7B1
Gene ID (NCBI) 9420
RRIDAB_2879780
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP7B1(Cytochrome P450 7B1) is also named as 25-hydroxycholesterol 7-alpha-hydroxylase and belongs to the cytochrome P450 family. It catalyzes the first and rate-limiting step in the coonversion of cholesterol to bile acids and CYP7B1-mediated catalysis may play a role for control of the cellular levels of androgens, not only of estrogens.

Protocols

Product Specific Protocols
WB protocol for CYP7B1 antibody 24889-1-APDownload protocol
IHC protocol for CYP7B1 antibody 24889-1-APDownload protocol
IF protocol for CYP7B1 antibody 24889-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouse,ratWB

Free Radic Biol Med

ALDH2 deficiency exacerbates MCD-diet induced MASLD by modulating bile acid metabolism

Authors - Jun Luo
mouseWB

Cell Physiol Biochem

Ganoderma Lucidum Polysaccharide Peptide Alleviates Hepatoteatosis via Modulating Bile Acid Metabolism Dependent on FXR-SHP/FGF.

Authors - Dandan Zhong
mouseWB

Mol Nutr Food Res

The Effect of Coenzyme Q10 Supplementation on Bile Acid Metabolism: Insights from Network Pharmacology, Molecular Docking, and Experimental Validation

Authors - Mengcheng Jin
mouseWB

J Nutr Biochem

Deficiency of coiled-coil domain containing 80 increases plasma cholesterol by decreasing fecal sterols excretion in hypercholesterolemic mice.

Authors - Wenyun Li
mouseWB

Biochem Pharmacol

Association between the perturbation of bile acid homeostasis and valproic acid-induced hepatotoxicity.

Authors - Yanan Chen
mouseWB

J Lipid Res

Insulin resistance dysregulates CYP7B1 leading to oxysterol accumulation: a pathway for NAFL to NASH transition.

Authors - Genta Kakiyama