CYP7B1 Polyclonal antibody

CYP7B1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

26

Cat no : 24889-1-AP

Synonyms

CBAS3, CP7B, CYP7B1, Cytochrome P450 7B1, Oxysterol 7 alpha hydroxylase, SPG5A

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Validation Data Gallery

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  WB analysis of A431 using 24889-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24889-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 24889-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse liver using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human brain using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24889-1-AP (CYP7B1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 24889-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24889-1-AP (CYP7B1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 24889-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYP7B1 antibody (24889-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  IF Staining of HEK-293 using 24889-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYP7B1 antibody (24889-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HEK-293 using 24889-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CYP7B1 antibody (24889-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, human brain tissue, human liver tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 25 publications below
• IHC: See 2 publications below

Product Information

24889-1-AP targets CYP7B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYP7B1 fusion protein Ag19374
• Full Name: cytochrome P450, family 7, subfamily B, polypeptide 1
• Calculated Molecular Weight: 506 aa, 58 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC136574
• Gene Symbol: CYP7B1
• Gene ID (NCBI): 9420
• RRID: AB_2879780
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP7B1(Cytochrome P450 7B1) is also named as 25-hydroxycholesterol 7-alpha-hydroxylase and belongs to the cytochrome P450 family. It catalyzes the first and rate-limiting step in the coonversion of cholesterol to bile acids and CYP7B1-mediated catalysis may play a role for control of the cellular levels of androgens, not only of estrogens.

Publications

Species	Application	Title
human,mouse,rat	WB	Free Radic Biol Med; ALDH2 deficiency exacerbates MCD-diet induced MASLD by modulating bile acid metabolism; Authors - Jun Luo
mouse	WB	Cell Physiol Biochem; Ganoderma Lucidum Polysaccharide Peptide Alleviates Hepatoteatosis via Modulating Bile Acid Metabolism Dependent on FXR-SHP/FGF.; Authors - Dandan Zhong
mouse	WB	Mol Nutr Food Res; The Effect of Coenzyme Q10 Supplementation on Bile Acid Metabolism: Insights from Network Pharmacology, Molecular Docking, and Experimental Validation; Authors - Mengcheng Jin
mouse	WB	J Nutr Biochem; Deficiency of coiled-coil domain containing 80 increases plasma cholesterol by decreasing fecal sterols excretion in hypercholesterolemic mice.; Authors - Wenyun Li
mouse	WB	Biochem Pharmacol; Association between the perturbation of bile acid homeostasis and valproic acid-induced hepatotoxicity.; Authors - Yanan Chen
mouse	WB	J Lipid Res; Insulin resistance dysregulates CYP7B1 leading to oxysterol accumulation: a pathway for NAFL to NASH transition.; Authors - Genta Kakiyama