CYP7A1 Polyclonal antibody, PBS Only

CYP7A1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18054-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 18054-1-AP (same clone as 18054-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18054-1-AP (CYP7A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 18054-1-AP (same clone as 18054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18054-1-AP (CYP7A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 18054-1-AP (same clone as 18054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18054-1-AP (CYP7A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 18054-1-AP (same clone as 18054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18054-1-AP (CYP7A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 18054-1-AP (same clone as 18054-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18054-1-AP (CYP7A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

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Product Information

18054-1-PBS targets CYP7A1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYP7A1 fusion protein Ag12572
• Full Name: cytochrome P450, family 7, subfamily A, polypeptide 1
• Calculated Molecular Weight: 504 aa, 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC101777
• Gene Symbol: CYP7A1
• Gene ID (NCBI): 1581
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CYP7A1 (Cytochrome P450 Family 7 Subfamily A Member 1), is a cholesterol 7alpha-hydroxylase, catalyzing the first and rate-limiting step in the neutral or classic pathway for bile acid biosynthesis. CYP7A1 is a postulated gene modifier of colorectal cancer risk and target for the therapeutic bile acid, ursodeoxycholic acid (UDCA). The CYP7A1 enzyme controls the rate-limiting step of the catabolism of cholesterol into bile acids in the liver.