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CYP51A1 Recombinant antibody, PBS Only

CYP51A1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242166G9

Cat no : 84705-4-PBS

Synonyms

Cytochrome P450 14DM, CYPLI, CYPL1, CYP51, CP51

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CYP51A1 Recombinant antibody (84705-4-RR)

    WB analysis using 84705-4-RR (same clone as 84705-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84705-4-RR (CYP51A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84705-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84705-4-RR

    Affinity and Kinetic Characterization of 84705-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84705-4-RR against Human CYP51A1 were performed. The affinity constant is below 1 pM.

Product Information

84705-4-PBS targets CYP51A1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CYP51A1 fusion protein Ag4235
Full Name cytochrome P450, family 51, subfamily A, polypeptide 1
Calculated Molecular Weight 509 aa, 57 kDa
Observed Molecular Weight56 kDa
GenBank Accession NumberBC032322
Gene Symbol CYP51A1
Gene ID (NCBI) 1595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Lanosterol 14-alpha demethylase (cytochrome P450(51), CYP51A1) belongs to the evolutionarily conserved family of cytochrome P450 and catalyzes one of the key steps in cholesterol biosynthesis. CYP51A1 protein is targeted for degradation when exposed to nitric oxide generated under inflammatory conditions by NOS2 or released from NO donor compounds (PMID: 28830911). CYP51A1 is a prognostic marker in certain cancers, including Cervical squamous cell carcinoma, Head and neck squamous cell carcinoma, Kidney chromophobe, and Kidney renal clear cell carcinoma (PMID: 24711211).