CYP3A5 Polyclonal antibody

CYP3A5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13737-1-AP

Synonyms

CP35, CYP3A5, CYPIIIA5, Cytochrome P450 3A5, Cytochrome P450 HLp2, Cytochrome P450 PCN3, P450PCN3, PCN3

---

Validation Data Gallery

• 

  WB analysis of mouse liver using 13737-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13737-1-AP (CYP3A5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13737-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13737-1-AP (CYP3A5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13737-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13737-1-AP (CYP3A5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13737-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13737-1-AP (CYP3A5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 13737-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13737-1-AP (CYP3A5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13737-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13737-1-AP (CYP3A5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse liver tissue, human liver tissue, HEK-293 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

13737-1-AP targets CYP3A5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYP3A5 fusion protein Ag4674
• Full Name: cytochrome P450, family 3, subfamily A, polypeptide 5
• Calculated Molecular Weight: 502 aa, 57 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC033862
• Gene Symbol: CYP3A5
• Gene ID (NCBI): 1577
• RRID: AB_2090516
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Front Oncol; Novel Prognostic Signatures of Hepatocellular Carcinoma Based on Metabolic Pathway Phenotypes.; Authors - Tingbo Ye
		Oncotarget; Alpha-ketoglutarate suppresses the NF-κB-mediated inflammatory pathway and enhances the PXR-regulated detoxification pathway.; Authors - Liuqin He
human	WB	Int J Mol Sci; Gene Expression Reprogramming by Citrate Supplementation Reduces HepG2 Cell Migration and Invasion; Authors - Rocchina Miglionico