CYP2F1 Polyclonal antibody

CYP2F1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21579-1-AP

Synonyms

C2F1, CYP2F, CYP2F1, CYPIIF1, Cytochrome P450 2F1

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Validation Data Gallery

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  WB analysis of mouse lung using 21579-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21579-1-AP (CYP2F1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 21579-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21579-1-AP (CYP2F1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 21579-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21579-1-AP (CYP2F1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 21579-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21579-1-AP (CYP2F1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse lung tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21579-1-AP is CYP2F1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYP2F1 fusion protein Ag16124
• Full Name: cytochrome P450, family 2, subfamily F, polypeptide 1
• Calculated Molecular Weight: 491 aa, 56 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC109056
• Gene Symbol: CYP2F1
• Gene ID (NCBI): 1572
• RRID: AB_10733220
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.