CYP2D6 Monoclonal antibody, PBS Only

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  WB analysis of HepG2 using 60297-1-Ig (same clone as 60297-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60297-1-Ig (CYP2D6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60297-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 60297-1-Ig (same clone as 60297-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60297-1-Ig (CYP2D6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60297-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60297-1-Ig (same clone as 60297-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60297-1-Ig (CYP2D6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60297-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60297-1-Ig (same clone as 60297-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60297-1-Ig (CYP2D6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60297-1-PBS in a different storage buffer formulation.

CYP2D6 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 60297-1-PBS

Clone No.8B11D12

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

CPD6, CYP2D, CYP2D@, CYP2D6, CYP2DL1, Cytochrome P450 2D6, P450 DB1, P450C2D, P450DB1

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Product Information

60297-1-PBS targets CYP2D6 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CYP2D6 fusion protein Ag12605
• Full Name: cytochrome P450, family 2, subfamily D, polypeptide 6
• Calculated Molecular Weight: 497 aa, 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC075023
• Gene Symbol: CYP2D6
• Gene ID (NCBI): 1565
• RRID: AB_2881412
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P10635
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

CYP2D6(Cytochrome P450 2D6) is also named as CYP2DL1 and belongs to the cytochrome P450 family. It is a monooxygenase that involved in the metabolism of drugs such as antiarrhythmics, adrenoceptor antagonists, and tricyclic antidepressants. CYP2D6 is responsible for the metabolism of many drugs and environmental chemicals that it oxidizes. It has 2 isoforms produced by alternative splicing with the molecular weight of 56 kDa and 50 kDa.