CYP27A1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CYP27A1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3G3F7

Cat no : 67045-1-PBS

Synonyms

CP27, CTX, CYP27, CYP27A1, Cytochrome P 450C27/25, Cytochrome P450 27, Sterol 27 hydroxylase, Vitamin D(3) 25 hydroxylase

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Validation Data Gallery

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  WB analysis of Caco-2 using 67045-1-Ig (same clone as 67045-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67045-1-Ig (same clone as 67045-1-PBS)

  WB result of CYP27A1 antibody (67045-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP27A1 transfected HepG2 cells. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67045-1-Ig (same clone as 67045-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-29 using 67045-1-Ig (same clone as 67045-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 67045-1-Ig (same clone as 67045-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67045-1-Ig (same clone as 67045-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-29 using 67045-1-Ig (same clone as 67045-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67045-1-Ig (CYP27A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67045-1-Ig (CYP27A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CYP27A1 antibody (67045-1-Ig, Clone: 3G3F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CYP27A1 antibody (67045-1-Ig, Clone: 3G3F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

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Product Information

67045-1-PBS targets CYP27A1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CYP27A1 fusion protein Ag7746
• Full Name: cytochrome P450, family 27, subfamily A, polypeptide 1
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 54-60 kDa
• GenBank Accession Number: BC051851
• Gene Symbol: CYP27A1
• Gene ID (NCBI): 1593
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.