CYP27A1 Polyclonal antibody

CYP27A1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

20

Reviews (1)

Cat no : 14739-1-AP

Synonyms

CP27, CTX, CYP27, CYP27A1, Cytochrome P 450C27/25, Cytochrome P450 27, Sterol 27 hydroxylase, Vitamin D(3) 25 hydroxylase

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Validation Data Gallery

• 

  WB analysis of mouse liver using 14739-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14739-1-AP (CYP27A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 14739-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14739-1-AP (CYP27A1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 14739-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14739-1-AP (CYP27A1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14739-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14739-1-AP (CYP27A1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14739-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14739-1-AP (CYP27A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 14739-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14739-1-AP (CYP27A1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue, NIH/3T3 cells
• Positive IHC detected in: human liver cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 17 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Product Information

14739-1-AP targets CYP27A1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, rabbit, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYP27A1 fusion protein Ag6408
• Full Name: cytochrome P450, family 27, subfamily A, polypeptide 1
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC051851
• Gene Symbol: CYP27A1
• Gene ID (NCBI): 1593
• RRID: AB_2089276
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mitochondrial sterol 27-hydroxylase (CYP27A1) catalyzes oxidative cleavage of the sterol side chain in the bile acid biosynthetic pathway in the liver and 27-hydroxylation of cholesterol in most tissues(PMID:17088262). It belongs to the cytochrome P450 family. CYP27A1 catalyses an important sterol elimination pathway in the macrophage, and consequently may protect against atherosclerosis.

Publications

Species	Application	Title
human	IHC	Cell Res; Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer.; Authors - Shuaishuai Teng
	IHC	J Lipid Res; Increased maternal and fetal cholesterol efflux capacity and placental CYP27A1 expression in preeclampsia.; Authors - Hiten D Mistry
rat	WB	Phytomedicine; Dehydrodiconiferyl alcohol, a lignan from Herpetospermum pedunculosum, alleviates cholestasis by activating pathways associated with the farnesoid X receptor.; Authors - Xiaodong Wei
rat	WB	Phytomedicine; Dolomiaea souliei ethyl acetate extract protected against α-naphthylisothiocyanate-induced acute intrahepatic cholestasis through regulation of farnesoid x receptor-mediated bile acid metabolism; Authors - FanCheng Meng
human,mouse	WB,IHC	Phytomedicine; Alisol B 23-acetate adjusts bile acid metabolisim via hepatic FXR-BSEP signaling activation to alleviate atherosclerosis.; Authors - Yu Fu
	WB	J Agric Food Chem; Oryzanol Attenuates High Fat and Cholesterol Diet-Induced Hyperlipidemia by Regulating the Gut Microbiome and Amino Acid Metabolism.; Authors - Sisi Yan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

kishu (Verified Customer) (06-10-2021)

got good results in WB for human and rat liver cells

• Applications: Western Blot
• Cell Tissue Type: liver cells