CYP26B1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CYP26B1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

17

Reviews (1)

Cat no : 21555-1-AP

Synonyms

Cytochrome P450RAI-2, Cytochrome P450RAI 2, Cytochrome P450 retinoic acid-inactivating 2, Cytochrome P450 26B1, Cytochrome P450 26A2

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Validation Data Gallery

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  WB analysis of human brain using 21555-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21555-1-AP (CYP26B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 21555-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21555-1-AP (CYP26B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 21555-1-AP

  IP result of anti-CYP26B1 (IP:21555-1-AP, 4ug; Detection:21555-1-AP 1:500) with L02 cells lysate 2400ug.

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  IHC staining of human hepatocirrhosis using 21555-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21555-1-AP (CYP26B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 21555-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21555-1-AP (CYP26B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 21555-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21555-1-AP (CYP26B1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 21555-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21555-1-AP (CYP26B1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 21555-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CYP26B1 antibody (21555-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, L02 cells
• Positive IP detected in: L02 cells
• Positive IHC detected in: human hepatocirrhosis tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 7 publications below
• IF: See 3 publications below

Product Information

21555-1-AP targets CYP26B1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYP26B1 fusion protein Ag15368
• Full Name: cytochrome P450, family 26, subfamily B, polypeptide 1
• Calculated Molecular Weight: 512 aa, 58 kDa
• Observed Molecular Weight: 49-58 kDa
• GenBank Accession Number: BC069443
• Gene Symbol: CYP26B1
• Gene ID (NCBI): 56603
• RRID: AB_10733471
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP26B1(Cytochrome P450 26B1) is involved in the metabolism of retinoic acid (RA), rendering this classical morphogen inactive through oxidation.It has 3 isoforms produced by alternative splicing.Cyp26b1 deficiency induces pronounced apoptosis in the developing limb and delayed chondrocyte maturation(PMID:15030763).Defects in CYP26B1 are the cause of radiohumeral fusions with other skeletal and craniofacial anomalies (RHFCA).

Publications

Species	Application	Title
human	WB	Nat Genet; Exome-wide analyses identify low-frequency variant in CYP26B1 and additional coding variants associated with esophageal squamous cell carcinoma.; Authors - Jiang Chang ; KD Validated
mouse	IHC	Brain Struct Funct; Activity-dependent and graded BACE1 expression in the olfactory epithelium is mediated by the retinoic acid metabolizing enzyme CYP26B1.; Authors - Hande Login
mouse	IF	J Neurosci; The Stimulus-Dependent Gradient of Cyp26B1+ Olfactory Sensory Neurons Is Necessary for the Functional Integrity of the Olfactory Sensory Map.; Authors - Hande Login
mouse	IF	J Neurosci; Increased Retinoic Acid Catabolism in Olfactory Sensory Neurons Activates Dormant Tissue-Specific Stem Cells and Accelerates Age-Related Metaplasia.; Authors - Sofia Håglin
		Front Mol Neurosci; Transcriptomics of Environmental Enrichment Reveals a Role for Retinoic Acid Signaling in Addiction.; Authors - Yafang Zhang
human	WB	FASEB J; Dysregulated retinoic acid signaling in airway smooth muscle cells in asthma.; Authors - Amy E Defnet

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Daniel (Verified Customer) (02-06-2020)

The antibody stains a subset of proximal tubulues.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: mouse kidney

CYP26B1 Antibody Immunofluorescence, validation (1:200 dilution) in mouse kidney (Cat no:21555-1-AP)