CYP24A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CYP24A1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

18

Reviews (1)

Cat no : 21582-1-AP

Synonyms

Cytochrome P450 24A1, CYP24, CP24, 24-OHase, 24 OHase

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Validation Data Gallery

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  WB analysis of A549 using 21582-1-AP

  WB result of CYP24A1 antibody (21582-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP24A1 transfected A549 cells.

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  WB analysis using 21582-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21582-1-AP (CYP24A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21582-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21582-1-AP (CYP24A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 21582-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21582-1-AP (CYP24A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 21582-1-AP

  IP result of anti-CYP24A1 (IP:21582-1-AP, 4ug; Detection:21582-1-AP 1:300) with HeLa cells lysate 2800ug.

• 

  IHC staining of human kidney using 21582-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21582-1-AP (CYP24A1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 21582-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21582-1-AP (CYP24A1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 21582-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 21582-1-AP (CYP24A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 21582-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 21582-1-AP (CYP24A1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human kidney tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 18 publications below
• IHC: See 5 publications below

Product Information

21582-1-AP targets CYP24A1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYP24A1 fusion protein Ag16134
• Full Name: cytochrome P450, family 24, subfamily A, polypeptide 1
• Calculated Molecular Weight: 514 aa, 59 kDa
• Observed Molecular Weight: 54-59 kDa
• GenBank Accession Number: BC109083
• Gene Symbol: CYP24A1
• Gene ID (NCBI): 1591
• RRID: AB_10792804
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP24A1(1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial) catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D3) and 1-alpha,25-dihydroxyvitamin D3. Cholecalciferol is metabolised by the 25-hydroxylases (CYP2R1 and CYP27A1) in the liver, before 25-hydroxycholecalciferol (25(OH)D3) relocates to the circulation (PMID:20362668). The gene CYP24A1 has three isoforms, for the transit peptide "mitochondrial domain" removed, the MW of CYP24A1 will be 55-59 kDa, 47-51 kDa and 43 kDa. This antibody is specific to CYP24A1.

Publications

Species	Application	Title
human	WB,IHC	Cancer Lett; TAZ regulates cell proliferation and sensitivity to vitamin D3 in intrahepatic cholangiocarcinoma.; Authors - Heng Xiao ; KD Validated
mouse	WB	Acta Physiol (Oxf); Intestinal epithelial ablation of Pit-2/Slc20a2 in mice leads to sustained elevation of vitamin D3 upon dietary restriction of phosphate.; Authors - Eva Maria Pastor-Arroyo
mouse	WB	J Bone Miner Res; Exercise May Ameliorate the Detrimental Side Effects of High Vitamin D Supplementation on Muscle Function in Mice.; Authors - Danielle A Debruin
human	WB	Cancer Commun (Lond); Squalene epoxidase promotes colorectal cancer cell proliferation through accumulating calcitriol and activating CYP24A1-mediated MAPK signaling.; Authors - Luwei He
human	WB, IHC	J Steroid Biochem Mol Biol; Eldecalcitol (ED-71), an Analog of 1α, 25-dihydroxy Vitamin D3 as a Potential Anti-cancer Agent for Oral Squamous Cell Carcinomas.; Authors - T Shintani
mouse	WB	J Physiol; 1,25(OH)2 vitamin D3 stimulates active phosphate transport but not paracellular phosphate absorption in mouse intestine.; Authors - Nati Hernando

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kenzo (Verified Customer) (08-30-2023)

This antibody appeared to work pretty well for immunofluorescence in mouse tissues.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Kidney