Various lysates were subjected to SDS PAGE followed by western blot with 67421-1-Ig (CYP21A2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 67421-1-Ig (CYP21A2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis of PC-12 using 67421-1-Ig
PC-12 cells were subjected to SDS PAGE followed by western blot with 67421-1-Ig (CYP21A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
PC-12 cells were subjected to SDS PAGE followed by western blot with 67421-1-Ig (CYP21A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of pig adrenal gland using 67421-1-Ig
pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67421-1-Ig (CYP21A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67421-1-Ig (CYP21A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IF Staining of PC-12 using 67421-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using CYP21A2 antibody (67421-1-Ig, Clone: 1G2G6 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using CYP21A2 antibody (67421-1-Ig, Clone: 1G2G6 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
Various lysates were subjected to SDS PAGE followed by western blot with 67421-1-Ig (CYP21A2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis of PC-12 using 67421-1-Ig
PC-12 cells were subjected to SDS PAGE followed by western blot with 67421-1-Ig (CYP21A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of pig adrenal gland using 67421-1-Ig
pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67421-1-Ig (CYP21A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IF/ICC Figures
IF Staining of PC-12 using 67421-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using CYP21A2 antibody (67421-1-Ig, Clone: 1G2G6 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CYP21A2 Monoclonal antibody
Catalog Number
67421-1-Ig
Citations
-
Dilutions
WB : 1:2000-1:10000 IF/ICC : 1:400-1:1600
Applications
WB, IF/ICC, ELISA
Reactivity
human, pig
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
