CYP20A1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CYP20A1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16702-1-AP

Synonyms

CYP M, CYP20A1, Cytochrome P450 20A1

---

Validation Data Gallery

• 

  WB analysis using 16702-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16702-1-AP (CYP20A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16702-1-AP

  WB result of CYP20A1 antibody (16702-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP20A1 transfected HEK-293 cells.

• 

  WB analysis of mouse lung using 16702-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16702-1-AP (CYP20A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 16702-1-AP

  IP result of anti-CYP20A1 (IP:16702-1-AP, 4ug; Detection:16702-1-AP 1:300) with MCF-7 cells lysate 2400 ug.

• 

  IHC staining of human liver cancer using 16702-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16702-1-AP (CYP20A1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 16702-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16702-1-AP (CYP20A1 antibody) at dilution of 1:200 (under 40x lens).

---

Tested Applications

• Positive WB detected in: HeLa cells, mouse lung tissue, HEK-293 cells, K-562 cells, MCF-7 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16702-1-AP targets CYP20A1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYP20A1 fusion protein Ag10099
• Full Name: cytochrome P450, family 20, subfamily A, polypeptide 1
• Calculated Molecular Weight: 462 aa, 52 kDa
• Observed Molecular Weight: 45-52 kDa
• GenBank Accession Number: BC020616
• Gene Symbol: CYP20A1
• Gene ID (NCBI): 57404
• RRID: AB_2261395
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.