CYP17A1 Polyclonal antibody

CYP17A1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

44

Reviews (3)

Cat no : 14447-1-AP

Synonyms

Cytochrome P450 C17, Cytochrome P450 17A1, CYPXVII, CYP17, CPT7

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Validation Data Gallery

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  WB analysis using 14447-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14447-1-AP (CYP17A1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 14447-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14447-1-AP (CYP17A1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14447-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14447-1-AP (CYP17A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 14447-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14447-1-AP (CYP17A1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human prostate cancer using 14447-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14447-1-AP (CYP17A1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human prostate cancer using 14447-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14447-1-AP (CYP17A1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human prostate cancer using 14447-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14447-1-AP (CYP17A1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 14447-1-AP

  Immunofluorescent analysis of HepG2 cells, using CYP17A1 antibody 14447-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HepG2 using 14447-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CYP17A1 Polyclonal antibody (14447-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A549 cells, Jurkat cells, rat brain tissue, HeLa cells, HepG2 cells, mouse adrenal gland tissue, mouse brain tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 23 publications below
• IHC: See 14 publications below
• IF: See 14 publications below

Product Information

14447-1-AP targets CYP17A1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYP17A1 fusion protein Ag5899
• Full Name: cytochrome P450, family 17, subfamily A, polypeptide 1
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 48-51 kDa
• GenBank Accession Number: BC062997
• Gene Symbol: CYP17A1
• Gene ID (NCBI): 1586
• RRID: AB_2292527
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The human cytochrome P450c17 enzyme is encoded by the CYP17A1 gene. It is the qualitative regulator of steroidogenesis in the human adrenals.In its absence, aldosterone is produced in the human adrenal zona glomerulosa .The CYP17A1 gene is expressed in the adrenal cortex and the gonads but not in the placenta (PMID;19403566). Although CYP17A1 has been shown to be a 50-kDa protein, the presence of a lower molecular mass(30 kDa) immunoreactive form in rat Leydig cells was previously shown and it is either a degradation product or a truncated form of the 50 kDa enzyme(PMID:15761033). Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) and CYP17A1 is an important target for inhibition in the treatment of prostate cancer (PMID 27505042, PMID 27505042).

Publications

Species	Application	Title
mouse	WB	Part Fibre Toxicol; Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway.; Authors - Haibo Jin
human	IHC	J Clin Endocrinol Metab; PMID: 25137424; Authors - Kaisa Huhtinen
mouse	IHC	J Cell Sci; Transgenic GATA-4 expression induces adrenocortical tumorigenesis in C57Bl/6 mice.; Authors - Chrusciel Marcin M
mouse	IHC	FASEB J; Hydroxysteroid (17β)-dehydrogenase 1-deficient female mice present with normal puberty onset but are severely subfertile due to a defect in luteinization and progesterone production.; Authors - Janne Hakkarainen
mouse	IF	Development; WNT signaling in pre-granulosa cells is required for ovarian folliculogenesis and female fertility.; Authors - Okiko Habara
rat	WB	Food Chem Toxicol; 3-Chloro-1, 2-propanediol inhibits autophagic flux by impairment of lysosomal function in HepG2 cells.; Authors - Jing Lu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (04-26-2024)

The size of the protein that the antibody recognizes in samples is much smaller than expected.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse liver and mouse brain

CYP17A1 Antibody Western Blot validation (1:1000 dilution) in mouse liver and mouse brain (Cat no:14447-1-AP)

FH

Tom (Verified Customer) (08-25-2021)

2 non-specific bands appear on membrane, but the correct band can be distinguished.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500

CYP17A1 Antibody Western Blot validation (1:500 dilution) in (Cat no:14447-1-AP)

FH

Hailey (Verified Customer) (10-07-2019)

This antibody works very well for IF in mouse testis. We use Cyp17a1 as a marker for a lot of my experiments, and after comparing Cyp17a1 antibodies from many companies, this is our antibody of choice for IF. However for westerns, there is a pesky non-specific band that appears right below the correct band, and it is difficult to differentiate them unless you run the WB for a long time.

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse testis

CYP17A1 Antibody Western Blot,Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Mouse testis (Cat no:14447-1-AP)