CYP11B2 Polyclonal antibody

CYP11B2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 20968-1-AP

Synonyms

CYP11BL, CYP11B, CPN2, Corticosterone 18-monooxygenase, CYP11B2, Aldosterone-synthesizing enzyme

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Validation Data Gallery

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  WB analysis using 20968-1-AP

  pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 20968-1-AP (CYP11B2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 20968-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 20968-1-AP (CYP11B2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human adrenal gland using 20968-1-AP

  Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue slide using 20968-1-AP (CYP11B2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human adrenal gland using 20968-1-AP

  Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue slide using 20968-1-AP (CYP11B2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20968-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 20968-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 20968-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 20968-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 20968-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 20968-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 20968-1-AP (CYP11B2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of SKOV-3 using 20968-1-AP

  Immunofluorescent analysis of SKOV-3 cells using 20968-1-AP (CYP11B2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SKOV-3 cells, pig adrenal gland tissue
• Positive IHC detected in: human adrenal gland tissue, human kidney tissue, human hepatocirrhosis tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SKOV-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

20968-1-AP targets CYP11B2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: cytochrome P450, family 11, subfamily B, polypeptide 2
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: NM_000498
• Gene Symbol: CYP11B2
• Gene ID (NCBI): 1585
• RRID: AB_2878777
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP11B2 (Cytochrome P450 11B2), also known as aldosterone synthase, is a member of the cytochrome P450 superfamily of enzymes. CYP11B2 can only catalyze 11-deoxycorticosterone oxidation to produce corticosterone and further hydroxylation to ALD. CYP11B2 is an enzyme required for cortisol biosynthesis and is specifically expressed in aldosterone-producing tissue of the adrenal cortex and is overexpressed in aldosterone-producing adenomas (APA).

Publications

Species	Application	Title
human	IHC	Nat Commun; Adrenal tropism of SARS-CoV-2 and adrenal findings in a post-mortem case series of patients with severe fatal COVID-19.; Authors - Tanja Paul
human	WB,IHC	Cell Death Dis; Aldosterone from endometrial glands is benefit for human decidualization.; Authors - Shu-Yun Li
mouse	IF	PeerJ; Adrenal SGLT1 or SGLT2 as predictors of atherosclerosis under chronic stress based on a computer algorithm; Authors - Jianyi Li