CYP11A1 Polyclonal antibody

CYP11A1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

52

Cat no : 13363-1-AP

Synonyms

Cytochrome P450(scc), Cytochrome P450 11A1, CYPXIA1, CYP11A, Cholesterol side-chain cleavage enzyme, mitochondrial

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Validation Data Gallery

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  WB analysis using 13363-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13363-1-AP (CYP11A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 13363-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13363-1-AP (CYP11A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13363-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13363-1-AP (CYP11A1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 13363-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13363-1-AP (CYP11A1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 13363-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13363-1-AP (CYP11A1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 13363-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13363-1-AP (CYP11A1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 13363-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13363-1-AP (CYP11A1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human placenta tissue, HeLa cells, mouse ovary tissue, mouse testis tissue, rat testis tissue
• Positive IHC detected in: human placenta tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 45 publications below
• IHC: See 5 publications below
• IF: See 5 publications below

Product Information

13363-1-AP targets CYP11A1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CYP11A1 fusion protein Ag4182
• Full Name: cytochrome P450, family 11, subfamily A, polypeptide 1
• Calculated Molecular Weight: 521 aa, 60 kDa
• Observed Molecular Weight: 49-52 kDa
• GenBank Accession Number: BC032329
• Gene Symbol: CYP11A1
• Gene ID (NCBI): 1583
• RRID: AB_2088552
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CYP11A1 is also known as cytochrome P450C11A1, cytochrome P450scc and cytochrome P450, subfamily XIA. It is the first enzyme of all steroidogenic pathways, its elimination results in syndromes of steroid deficiency, including electrolyte imbalance, aberrant gene regulation, and suppression of male sex organ development(PMID: 12145347). Expression of CYP11A1 has been a useful tool for monitoring the differentiation state of cells from varius endocrine tissues. Identification of CYP11A1 expression by 13363-1-AP detected a ~45kd band in human placenta, with respect to the reported band at 49-52kd by Roby, et al and Durkee, et al. The murine 32-kDa isoform of Cyp11a1 is not produced by omission of the first ATG-containing exon (as is the case for human CYP11A1) but perhaps by alternative translational initiation(PMID:19342447).

Publications

Species	Application	Title
rat	WB	Science; Pregnenolone can protect the brain from cannabis intoxication.; Authors - Monique Vallée
mouse	WB	Brain Behav Immun; Placenta-specific CYP11A1 overexpression lead to autism-like symptom in offspring with altered steroid hormone biosynthesis in the placenta-brain axis and rescued by vitamin D intervention; Authors - Heng Yin
human	WB	Nat Commun; 1,25D3 prevents CD8(+)Tc2 skewing and asthma development through VDR binding changes to the Cyp11a1 promoter.; Authors - Michaela Schedel
mouse	WB	Autophagy; Epg5 deficiency leads to primary ovarian insufficiency due to WT1 accumulation in mouse granulosa cells.; Authors - Wenwen Liu
human	WB,IHC	Clin Transl Med; Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.; Authors - Lijie Zhou
mouse	WB	Part Fibre Toxicol; Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway.; Authors - Haibo Jin