KK-LC-1 Polyclonal antibody

KK-LC-1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 25708-1-AP

Synonyms

Cancer/testis antigen 83, CT83, CXorf61, KK LC 1, KKLC1, KK-LC-1

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Validation Data Gallery

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  WB analysis using 25708-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25708-1-AP (KK-LC-1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 25708-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25708-1-AP (KK-LC-1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 25708-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25708-1-AP (KK-LC-1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 25708-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25708-1-AP (KK-LC-1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 25708-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25708-1-AP (KK-LC-1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: human placenta tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 3 publications below

Product Information

25708-1-AP targets KK-LC-1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KK-LC-1 fusion protein Ag22619
• Full Name: chromosome X open reading frame 61
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC062223
• Gene Symbol: KK-LC-1
• Gene ID (NCBI): 203413
• RRID: AB_2880203
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KK-LC-1 has been described as kita-kyushu lung cancer antigen 1, cancer/testis antigen 83. Therefore, KK-LC-1 is also known as CXorf61 and CT83. KK-LC-1 has been tested for association to diseases, such as adenocarcinoma and lung neoplasms. The molecular mass of KK-LC-1 is 13 kDa. Studies have shown that KK-LC-1 is an ideal target for immunotherapy of triple-negative breast cancer (TNBC) (PMID: 26327325).

Publications

Species	Application	Title
human	IHC	Int Immunopharmacol; Integrative analysis-based identification and validation of a prognostic immune cell infiltration-based model for patients with advanced gastric cancer.; Authors - Siwei Pan
mouse	WB,IHC	Nat Commun; KK-LC-1 as a therapeutic target to eliminate ALDH+ stem cells in triple negative breast cancer; Authors - Jiawen Bu
human	IHC	Cancer Immunol Immunother; Integrating bulk and single-cell sequencing data to construct a Scissor+ dendritic cells prognostic model for predicting prognosis and immune responses in ESCC; Authors - Maosheng Cheng