CXorf21 Polyclonal antibody

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  WB analysis using 30244-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 30244-1-AP (CXorf21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skin using 30244-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 30244-1-AP (CXorf21 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 30244-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 30244-1-AP (CXorf21 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat skin using 30244-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 30244-1-AP (CXorf21 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 30244-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 30244-1-AP (CXorf21 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat skin using 30244-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 30244-1-AP (CXorf21 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat stomach using 30244-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 30244-1-AP (CXorf21 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 30244-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CXorf21 antibody (30244-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U-251 using 30244-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using CXorf21 antibody (30244-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

CXorf21 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 30244-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TASL, TLR adapter interacting with SLC15A4 on the lysosome

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Tested Applications

• Positive WB detected in: THP-1 cells
• Positive IHC detected in: mouse skin tissue, human cervical cancer tissue, rat skin tissue, rat stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U-251 cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30244-1-AP targets CXorf21 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CXorf21 fusion protein Ag32773
• Full Name: chromosome X open reading frame 21
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC020611
• Gene Symbol: CXorf21
• Gene ID (NCBI): 80231
• RRID: AB_3086276
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HAI6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXorf21 (Chromosome X open reading frame 21) is also named as TASL and TLR adapter interacting with SLC15A4 on the lysosome. CXorf21 is an innate immune adapter that mediates the recruitment and activation of IRF5 downstream of endolysosomal toll-like receptors TLR7, TLR8 and TLR9 (PMID:32433612). CXorf21 plays a role in the regulation of endolysosomal pH in immune cells such as B-cells, dendritic cells and monocytes (PMID: 31001245). CXorf21 is a mediator of the X-chromosome gene dose-dependent increased risk of SLE in females (PMID: 31695690).