CXXC5 Recombinant antibody

CXXC5 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241034A6

Cat no : 83940-7-RR

Synonyms

Putative MAPK-activating protein PM08, HSPC195, CXXC-type zinc finger protein 5, CXXC finger 5, CF5

---

Validation Data Gallery

• 

  WB analysis using 83940-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83940-7-RR (CXXC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83940-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83940-7-RR against Human CXXC5 were performed. The affinity constant is 5.73 nM.

---

Tested Applications

• Positive WB detected in: MCF-7 cells, K-562 cells, mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83940-7-RR is CXXC5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CXXC5 fusion protein Ag9733
• Full Name: CXXC finger 5
• Calculated Molecular Weight: 322aa,33 kDa; 227aa,24 kDa
• Observed Molecular Weight: 33-40 kDa
• GenBank Accession Number: BC017439
• Gene Symbol: CXXC5
• Gene ID (NCBI): 51523
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXXC5 (CXXC-type zinc finger protein 5) is also named as HSPC195, RINF and TCCCIA00297.CXXC5 is a member of the CXXC-type zinc-finger protein family. CXXC5 is a short protein (322 amino acids in length) that does not have any catalytic domain, but is able to bind to DNA and act as a transcription factor and epigenetic factor through protein-protein interactions. In addition, CXXC5 is capable of regulating various signal transduction processes, including the TGF-β, Wnt and ATM-p53 pathways, thereby acting as a novel and crucial signaling coordinator. CXXC5 plays an important role in embryonic development and adult tissue homeostasis by regulating cell proliferation, differentiation and apoptosis (PMID: 30479059). CXXC5 has two isoforms of 33 kDa and 24 kDa (PMID: 25805812).