CXXC5 Polyclonal antibody

• Featured Product
• KD/KO Validated

CXXC5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 16513-1-AP

Synonyms

Putative NF-kappa-B-activating protein 102, Putative MAPK-activating protein PM08, HSPC195, CXXC-type zinc finger protein 5, CXXC finger 5

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Validation Data Gallery

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  WB analysis of mouse brain using 16513-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16513-1-AP (CXXC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16513-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16513-1-AP (CXXC5 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 16513-1-AP

  IP result of anti-CXXC5 (IP:16513-1-AP, 4ug; Detection:16513-1-AP 1:6000) with SH-SY5Y cells lysate 1280 ug.

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  IP experiment of SH-SY5Y using 16513-1-AP

  IP result of anti-CXXC5 (IP:16513-1-AP, 4ug; Detection:16513-1-AP 1:300) with SH-SY5Y cells lysate 3400 ug.

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  IP experiment of mouse brain using 16513-1-AP

  IP result of anti-CXXC5 (IP:16513-1-AP, 4ug; Detection:16513-1-AP 1:6000) with mouse brain tissue lysate 2400 ug.

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  IHC staining of human prostate cancer using 16513-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16513-1-AP (CXXC5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16513-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16513-1-AP (CXXC5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16513-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16513-1-AP (CXXC5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 16513-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16513-1-AP (CXXC5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 16513-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CXXC5 antibody (16513-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: MCF-7 cells, mouse brain tissue, SH-SY5Y cells
• Positive IP detected in: SH-SY5Y cells, mouse brain tissue
• Positive IHC detected in: mouse brain tissue, human prostate cancer tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• ChIP: See 4 publications below

Product Information

16513-1-AP targets CXXC5 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CXXC5 fusion protein Ag9733
• Full Name: CXXC finger 5
• Calculated Molecular Weight: 322aa,33 kDa; 227aa,24 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC017439
• Gene Symbol: CXXC5
• Gene ID (NCBI): 51523
• RRID: AB_2878269
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CXXC5 (CXXC-type zinc finger protein 5) is also named as HSPC195, RINF and TCCCIA00297.CXXC5 is a member of the CXXC-type zinc-finger protein family. CXXC5 is a short protein (322 amino acids in length) that does not have any catalytic domain, but is able to bind to DNA and act as a transcription factor and epigenetic factor through protein-protein interactions. In addition, CXXC5 is capable of regulating various signal transduction processes, including the TGF-β, Wnt and ATM-p53 pathways, thereby acting as a novel and crucial signaling coordinator. CXXC5 plays an important role in embryonic development and adult tissue homeostasis by regulating cell proliferation, differentiation and apoptosis (PMID: 30479059). CXXC5 has two isoforms of 33 kDa and 24 kDa (PMID: 25805812). CXXC5 is modified by phosphorylation after translation.

Publications

Species	Application	Title
human	ChIP,IHC,WB	Nat Commun; A noncanonical AR addiction drives enzalutamide resistance in prostate cancer.; Authors - Yundong He ; KD Validated
mouse	WB	J Exp Med; Epigenetic regulator CXXC5 recruits DNA demethylase Tet2 to regulate TLR7/9-elicited IFN response in pDCs.; Authors - Shixin Ma ; KO Validated
human	WB	Oncogene; NUDT21 negatively regulates PSMB2 and CXXC5 by alternative polyadenylation and contributes to hepatocellular carcinoma suppression.; Authors - Sheng Tan ; KD Validated
human	WB,ChIP	Haematologica; The epigenetic regulator RINF (CXXC5) maintains SMAD7 expression in human immature erythroid cells and sustains red blood cells expansion.; Authors - Audrey Astori
human	WB	Sci Rep; KANK1 inhibits cell growth by inducing apoptosis though regulating CXXC5 in human malignant peripheral nerve sheath tumors.; Authors - Zhibin Cui
human,mouse	WB,ChIP	Front Cell Dev Biol; Down-Regulation of CXXC5 De-Represses MYCL1 to Promote Hepatic Stellate Cell Activation.; Authors - Xiaoyan Wu