CXCR7 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CXCR7 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20423-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of Raji using 20423-1-AP (same clone as 20423-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 20423-1-AP (CXCR7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20423-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 20423-1-AP (same clone as 20423-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 20423-1-AP (CXCR7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20423-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 20423-1-AP (same clone as 20423-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20423-1-AP (CXCR7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20423-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 20423-1-AP (same clone as 20423-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20423-1-AP (CXCR7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20423-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HUVEC using 20423-1-AP (same clone as 20423-1-PBS)

  IP result of anti-CXCR7 (IP:20423-1-AP, 3ug; Detection:20423-1-AP 1:500) with HUVEC cells lysate 1400ug. This data was developed using the same antibody clone with 20423-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20423-1-AP (same clone as 20423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20423-1-AP (CXCR7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20423-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20423-1-AP (same clone as 20423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20423-1-AP (CXCR7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20423-1-PBS in a different storage buffer formulation.

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Product Information

20423-1-PBS targets CXCR7 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CXCR7 fusion protein Ag14247
• Full Name: chemokine (C-X-C motif) receptor 7
• Calculated Molecular Weight: 362 aa, 41 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC036661
• Gene Symbol: CXCR7
• Gene ID (NCBI): 57007
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a member of the G-protein coupled receptor family. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as coreceptor with CXCR4 for a restricted number of HIV isolates. Expression of CXCR7 has been associated with cardiac development as well as with tumor growth and progression. This antibody recognizes endogenous CXCR7, which has an experimentally determined molecular weight of 50 kDa (PMID: 20197403; 20388803).