CXCR4 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CXCR4 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4B5E4

Cat no : 60042-1-PBS

Synonyms

CD184, CXC R4, CXCR 4, CXCR4, CXCR4B, D2S201E, FB22, Fusin, HM89, HSY3RR, LAP3, LCR1, LESTR, SDF 1 receptor, WHIM

---

Validation Data Gallery

• 

  WB analysis of HeLa using 60042-1-Ig (same clone as 60042-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

• 

  WB analysis of THP-1 using 60042-1-Ig (same clone as 60042-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Ramos using 60042-1-Ig (same clone as 60042-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 60042-1-Ig (same clone as 60042-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U-937 using 60042-1-Ig (same clone as 60042-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 60042-1-Ig (same clone as 60042-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Daudi using 60042-1-Ig (same clone as 60042-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60042-1-Ig (same clone as 60042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60042-1-Ig (same clone as 60042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60042-1-Ig (same clone as 60042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60042-1-Ig (same clone as 60042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 60042-1-Ig (same clone as 60042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 60042-1-Ig (same clone as 60042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 60042-1-Ig (same clone as 60042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 60042-1-Ig (same clone as 60042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 60042-1-Ig (same clone as 60042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 60042-1-Ig (same clone as 60042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60042-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60042-1-PBS targets CXCR4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: CXCR4 fusion protein Ag1528
• Full Name: chemokine (C-X-C motif) receptor 4
• Calculated Molecular Weight: 352 aa, 40 kDa
• Observed Molecular Weight: 60~70 kDa
• GenBank Accession Number: BC020968
• Gene Symbol: CXCR4
• Gene ID (NCBI): 7852
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some researches, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).