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CXCL8/IL-8 Recombinant antibody, PBS Only

CXCL8/IL-8 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240889C4

Cat no : 83903-4-PBS

Synonyms

CXCL8, IL8, IL-8, C-X-C motif chemokine 8, C-X-C motif

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using CXCL8/IL-8 Recombinant antibody (83903-4-RR)

    WB analysis of THP-1 using 83903-4-RR (same clone as 83903-4-PBS)

    PMA, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 83903-4-RR (CXCL8,IL8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83903-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83903-4-RR

    Affinity and Kinetic Characterization of 83903-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83903-4-RR against Human CXCL8/IL-8 were performed. The affinity constant is 0.165 nM.

Product Information

The immunogen of 83903-4-PBS is CXCL8/IL-8 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name interleukin 8
Calculated Molecular Weight 99 aa, 11 kDa
Observed Molecular Weight11 kDa
GenBank Accession NumberBC013615
Gene Symbol IL-8
Gene ID (NCBI) 3576
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Interleukin 8 (IL-8), also known as CXCL8, is a CXC chemokine family member. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression. The human IL-8 cDNA sequence predicts a protein of 99 amino acids. Removal of a 22-residue signal peptide generates a mature protein of 77 amino acids (~ 8 kDa).